Device and method for treatment of surface infections with nitric oxide
First Claim
1. A method for debriding necrotic tissue located at a wound site of a subject, comprising:
- exposing the wound site to exogenous nitric oxide gas at a concentration and for a period of time sufficient to increase expression of endogenous collagenase at the wound site,wherein the concentration of exogenous nitric oxide gas is from about 120 ppm to about 400 ppm, andwherein the period of time is from about 5 hours to about 72 hours.
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Abstract
Topical exposure of nitric oxide gas to wounds such as chronic non-healing wounds may be beneficial in promoting healing of the wound and in preparing the wound bed for further treatment and recovery. Nitric oxide gas may be used, for example, to reduce the microbial infection and burden on these wounds, manage exudate secretion by reducing inflammation, upregulate expression of endogenous collagenase to locally debride the wound, and regulate the formation of collagen. High concentration of nitric oxide ranging from about 160 to 400 ppm may be used without inducing toxicity in the healthy cells around a wound site. Additionally, exposure to the high concentration for a first treatment period reduces the microbial burden and inflammation at the wound site and increase collagenase expression to debride necrotic tissue at the wound site. After a first treatment period with high concentration of nitric oxide, a second treatment period at a lower concentration of nitric oxide preferably ranging from about 5-20 ppm may to provided to restore the balance of nitric oxide and induce collagen expression to aid in the closure of the wound.
184 Citations
19 Claims
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1. A method for debriding necrotic tissue located at a wound site of a subject, comprising:
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exposing the wound site to exogenous nitric oxide gas at a concentration and for a period of time sufficient to increase expression of endogenous collagenase at the wound site, wherein the concentration of exogenous nitric oxide gas is from about 120 ppm to about 400 ppm, and wherein the period of time is from about 5 hours to about 72 hours. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for debriding necrotic tissue located at a wound site of a subject, comprising:
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providing a flow-controlled source of gas containing exogenous nitric oxide; and exposing the wound site to exogenous nitric oxide gas from the flow-controlled source of gas at a concentration and for a period of time sufficient to increase expression of endogenous collagenase at the wound site; wherein the increased expression of endogenous collagenase at the wound site contributes to debriding of the necrotic tissue; wherein the concentration of exogenous nitric oxide gas is from about 120 ppm to about 400 ppm; and wherein the period of time is from about 5 hours to about 72 hours. - View Dependent Claims (8, 9, 10, 11, 12, 13)
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14. A method of managing exudate from necrotic tissue at a wound site of a subject, comprising:
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exposing the wound site to exogenous nitric oxide gas at a concentration and for a period of time sufficient to reduce inflammation at the wound site, wherein the concentration of exogenous nitric oxide gas is from about 120 ppm to about 400 ppm, and wherein the period of time is from about 5 hours to about 72 hours. - View Dependent Claims (15, 16, 17, 18, 19)
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Specification