Apparatus and microchip for sorting micro particles

• US 8,795,500 B2
• Filed: 02/09/2010
• Issued: 08/05/2014
• Est. Priority Date: 02/17/2009
• Status: Active Grant

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Shinoda, Masataka
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US13/147,517
• Publication Number:
  US 20110284378A1
• Time in Patent Office: 1,638 Days
• Field of Search: 209576-581, 209/3, 209/3.1, 204/600, 204/601, 204/450, 204/451, 422502-504, 422/508
• US Class Current: 204/600
• CPC Class Codes: B01L 2400/0433 vibrational forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1409 Handling samples, e.g. injecting samples
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 15/1492 within droplets
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1028 Sorting particles
• CPC Class Codes: G01N 2015/1422 Electrical focussing
• CPC Class Codes: G01N 2015/1481 Optical analysis of particles within droplets sorting particles within droplets G01N15/1492
• CPC Class Codes: G01N 2015/1488 Methods for deciding
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10T 137/8158 : With indicator, register, recorder, alarm or inspection means

Abstract

In one example embodiment, a micro-particle sorting apparatus includes: (a) a microchip in which a flow path through which liquid containing a micro particle flows and an orifice through which the liquid flowing through the flow path is discharged into a space outside the chip; (b) an oscillating element for transforming the liquid into a liquid drop and discharging the liquid drop at the orifice; (c) a charge means for adding an electric charge to the discharged liquid drop; (d) an optical detection means that detects an optical property of the micro particle flowing through the flow path, upstream of a liquid-delivering direction with respect to the orifice; (e) paired electrodes opposed to each other while sandwiching the moving liquid drop along a movement direction of the liquid drop discharged into the space outside the chip; and (f) two containers that collect the liquid drop passing between the paired electrodes.

Claims

1. A micro-particle sorting apparatus comprising:

a microchip in which a flow path through which liquid containing a micro particle flows and an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip;

an oscillating element configured to transform the liquid into a liquid drop and discharge the liquid drop at the orifice;

a charge means for adding an electric charge to the discharged liquid drop;

an optical detection means that detects an optical property of the micro particle flowing through the flow path, upstream of a liquid-delivering direction with respect to the orifice;

paired electrodes provided so as to be opposed to each other while sandwiching the moving liquid drop therebetween along a movement direction of the liquid drop discharged into the space outside the microchip;

two or more containers that collect the liquid drop passing between the paired electrodes,

wherein a width of the flow path and a depth of the flow path at a location of the orifice are set to be smaller than a width of the flow path and a depth of the flow path at a location at which the optical property of the micro particle is detected by the optical detection means; and

wherein at least the orifice portion of the microchip and the space outside the microchip in which the liquid drop discharged through the orifice moves are arranged in a cavity of a cartridge having light transmittance for light from the optical detection means.

2. The micro-particle sorting apparatus of claim 1, which includes a micro tube that introduces, into a laminar flow of liquid T flowing through the flow path, a laminar flow of another liquid S containing the micro particle, upstream of the liquid-delivering direction with respect to the location at which the optical property of the micro particles is detected by the optical detection means.

3. The micro-particle sorting apparatus of claim 2, wherein the micro tube is formed of a metal on which voltage can be applied and is configured as the charge means.

4. The micro-particle sorting apparatus of claim 1, wherein the cavity of the cartridge is configured to be hermetically sealed.

5. A micro-particle sorting apparatus comprising:

a microchip in which a flow path through which liquid containing a micro particle flows and an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip;

an oscillating element for transforming the liquid into a liquid drop and discharging the liquid drop at the orifice;

a charge means for adding an electric charge to the discharged liquid drop;

an optical detection means that detects an optical property of the micro particle flowing through the flow path, upstream of a liquid-delivering direction with respect to the orifice; and

paired electrodes provided so as to be opposed to each other while sandwiching the moving liquid drop therebetween along a movement direction of the liquid drop discharged into the space outside the microchip; and

two or more containers that collect the liquid drop passing between the paired electrodes,

wherein a cross-section area of the flow path at a location of the orifice is set to be smaller than a cross-section area of the flow path at a location at which the optical property of the micro particle is detected by the optical detection means; and

wherein at least the orifice portion of the microchip and the space outside the microchip in which the liquid discharged through the orifice moves are arranged in a cavity of a cartridge having light transmittance for light from the optical detection mean.

6. A microchip comprising:

a flow path through which liquid containing a micro particle flows and an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip;

a predetermined location of the flow path configured as a light-irradiated portion to be irradiated with light from an optical detection means for detecting an optical property of the micro particle flowing therethrough; and

a micro tube that introduces, into a laminar flow of liquid T flowing through the flow path, a laminar flow of another liquid S containing micro particle, upstream of the liquid-delivering direction with respect to the light-irradiated portion, wherein a width of the flow path and a depth of the flow path at a location of the orifice are set to be smaller than a width of the flow path and a depth of the flow path at the light-irradiated portio; and

wherein the micro tube is formed of a metal on which voltage can be applied.

7. The microchip of claim 6, which includes an oscillating element for transforming the liquid into a liquid drop and discharging the liquid drop at the orifice.

8. A cartridge which has a cavity in which at least the orifice portion of the microchip of claim 6 and the space outside the microchip in which the liquid drop discharged through the orifice moves are arranged, wherein the cartridge has light transmittance with which light from the optical detection means is caused to transmit to the light-irradiated portion.

9. The cartridge of claim 8, wherein the cavity is configured to be hermetically sealed.

10. A microchip comprising:

a flow path through which liquid containing a micro particle flows and an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip;

a predetermined location of the flow path configured as a light-irradiated portion to be irradiated with light from an optical detection means for detecting an optical property of the micro particle flowing therethrough; and

a micro tube that introduces, into a laminar flow of liquid T flowing through the flow path, a laminar flow of another liquid S containing micro particle, upstream of the liquid-delivering direction with respect to the light-irradiated portion, wherein a cross-section area of the flow path at a location of the orifice is set to be smaller than a cross-section area of the flow path at the light-irradiated portion; and

wherein the micro tube is formed of a metal on which voltage can be applied.