Grating-based evanescent field molecular sensor using a thin silicon waveguide layer

• US 8,796,012 B2
• Filed: 12/02/2009
• Issued: 08/05/2014
• Est. Priority Date: 12/02/2008
• Status: Active Grant

• Current Assignee: National Research Council Canada
• Original Assignee: National Research Council Canada
• Inventors: Sinclair, Bill, Schmid, Jens, Waldron, Philip, Xu, Dan-Xia, Densmore, Adam, Mischki, Trevor, Lapointe, Jean, Poitras, Daniel, Janz, Siegfried, Lopinski, Greg
• Primary Examiner(s): Chin, Chris L
• Application Number:
  US13/129,892
• Publication Number:
  US 20110223688A1
• Time in Patent Office: 1,707 Days
• Field of Search: None
• US Class Current: 435/288.7
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/551 the carrier being inorganic
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

A technique for high sensitivity evanescent field molecular sensing employs a detection scheme that simultaneously couples a polarized beam to a single mode of a waveguide, and couples the polarized beam out of the waveguide to specularly reflect the beam by the same grating. Strong interaction with the single (preferably TM) mode is provided by using a silicon on insulator (SOI) wafer having a waveguide thickness chosen between 10-400 nm so that the majority of the mode field strength spans the evanescent field. Well known, robust techniques for producing a grating on the waveguide are provided. Interrogation from a backside of the SOI wafer is taught.

Claims

1. A molecular sensor comprising:

a silicon on insulator (SOI) wafer comprising a single crystal silicon layer waveguide over a SiO₂ insulator layer, the waveguide having a diffraction grating on a sensor surface which is functionalized with receptors for a specific target molecule;

such that the grating couples a polarized beam of light incident the sensor surface to excite a single mode in the waveguide and equally couples the single mode from the waveguide to form a sensed output signal; and

the waveguide has a thickness of 10-400 nm, selected so that an overlap integral of the waveguide'"'"'s mode intensity over the functionalized sensor surface is greater than 50% of the overlap integral over any other region of the same thickness within the waveguide.

2. The molecular sensor of claim 1 wherein the single mode has a peak evanescent field strength in the region of the sensor surface that is higher than the field strength at any point within the waveguide.

3. The molecular sensor of claim 1 wherein the thickness of the waveguide provides the single mode having a region of highest field strength within a distance from the sensor surface that corresponds to where a monolayer of the target molecules would be located.

4. The molecular sensor of claim 1 wherein the diffraction grating on the sensor surface is of a different composition than the silicon.

5. The molecular sensor of claim 1 wherein the diffraction grating on the sensor surface is substantially composed of a transparent polymer or inorganic compound.

6. The molecular sensor of claim 1 wherein the diffraction grating on the sensor surface is composed of silicon dioxide, or silicon nitride.

7. The molecular sensor of claim 1 wherein the diffraction grating on the sensor surface is a dielectric layer between 1 to 2000 nm thick, having a regular spacing matching a resonant wavelength of the single mode.

8. The molecular sensor of claim 1 wherein the diffraction grating on the sensor surface is formed by patterning the functionaiization of the sensor surface, by selective deposition of the receptors directly on the silicon waveguide surface.

9. The molecular sensor of claim 1 wherein the beam is incident the sensor surface from a bottom silicon layer of the SOI wafer.

10. The molecular sensor of claim 9 wherein the bottom insulator layer of the SOI is coated with an antireflection film.

11. The molecular sensor of claim 1 wherein the beam is P-polarized, and the single waveguide mode is a transverse magnetic (TM) mode.

12. The molecular sensor of claim 1 further comprising a detector for determining interference of a through-transmission beam or a compound specular reflection beam with the sensed output.

13. The molecular sensor of claim 1 further comprising a detector for detecting specular reflection power at a narrow wavelength band to effectively determine a shift in reflected or transmitted power of the beam that depends on the evanescent field interaction with the target molecules.

14. The molecular sensor of claim 1 further comprising a detector array for measuring a change in intensity of a diffracted ray of light.

15. The molecular sensor of claim 1 wherein the beam has a center wavelength of 1200 to 4000 nm, more preferably 1200-2000 nm, or one of 1280-1350 and 1480-1600 nm.

16. The molecular sensor of claim 1 wherein the waveguide is part of a silicon waveguide layer that extends beyond the grating, further comprising an array of like molecular sensors with similar or dissimilar functionalization.

17. A method of sensing a target molecule if present, within a fluid flow, the method comprising:

providing a silicon on insulator (SOI) wafer comprising a single crystal silicon layer waveguide over a SiO₂ insulator layer, the waveguide having a diffraction grating on a sensor surface that is functionalized with receptors for a specific target molecule;

applying the fluid flow to the sensor surface so that if the target molecule is present, it will preferentially attach to the receptors;

applying a polarized beam of light incident the sensor surface so that the diffraction grating couples the beam to excite a single mode in the waveguide and equally couples the single mode from the waveguide to form a sensed output signal, the waveguide having a thickness of 10-400 nm, selected so that art overlap integral of the waveguide'"'"'s mode intensity over the functionalized sensor surface is greater than 50% of the overlap integral over any other region of the same thickness within the waveguide; and

determining a property of the sensed output that varies with interaction of the evanescent field with the target molecules if present.

18. The method of claim 17 wherein providing the SOI wafer comprises providing the diffraction grating on the sensor surface formed by patterning the functionalization of the top sensor surface, by selective deposition of the receptors directly on the silicon waveguide surface.

19. The method of claim 17 wherein applying the beam comprises directing an incident beam onto the sensor surface from a bottom silicon layer of the SOI.

20. The method of claim 17 wherein applying the beam comprises applying P-polarized beam to selectively couple with the single mode, which is a transverse magnetic (TM) mode.

21. The method of claim 17 wherein the thickness of the waveguide provided ensures that the single mode has a region of highest field strength within a distance from the sensor surface that corresponds to where a monolayer of the target molecules would be located.

22. The method of claim 17 wherein determining the property comprises interfering the sensed output beam with a transmitted beam or specular reflection.

23. The method of claim 17 wherein determining the property comprises detecting a change in the power of a higher order diffraction of the beam, or computing a measure that varies therewith.