Ionization probe assemblies

• US 8,796,617 B2
• Filed: 01/03/2012
• Issued: 08/05/2014
• Est. Priority Date: 02/12/2009
• Status: Active Grant

• Current Assignee: Ibis Biosciences Incorporated
• Original Assignee: Ibis Biosciences Incorporated
• Inventors: Hofstadler, Steven A., Gutierrez, Jose R., Hannis, James C., Drader, Jared J., Bare, Rex O., Smith, Jeffrey C., Gleason, Paul J., Nathanson, Jared
• Primary Examiner(s): Wells, Nikita
• Application Number:
  US13/342,760
• Publication Number:
  US 20120097846A1
• Time in Patent Office: 945 Days
• Field of Search: 250/288, 250/281, 250/282, 250/425, 239/692
• US Class Current: 250/288
• CPC Class Codes: C12Q 1/6872 involving mass spectrometry
• CPC Class Codes: G01N 23/00 Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
• CPC Class Codes: G01R 31/2812 Checking for open circuits or shorts, e.g. solder bridges; Testing conductivity, resistivity or impedance of connections G01R31/66
• CPC Class Codes: G01R 31/2848 using simulation
• CPC Class Codes: G01R 31/2853 Electrical testing of internal connections or -isolation, e.g. latch-up or chip-to-lead connections G01R31/31717 takes precedence; test of chip-to-PCB or lead-to-PCB connections G01R31/66
• CPC Class Codes: H01J 27/02 Ion sources; Ion guns for examination or processing discharge tubes H01J37/08; ion sources, ion guns for particle spectrometer or separator tubes H01J49/10; ion propulsion F03H1/00
• CPC Class Codes: H01J 49/0422 for gaseous samples interfaces to gas chromatographs G01N30/7206
• CPC Class Codes: H01J 49/0431 for liquid samples interfaces to liquid chromatographs G01N30/7233
• CPC Class Codes: H01J 49/0468 with means for heating or cooling the sample
• CPC Class Codes: H01J 49/165 Electrospray ionisation

Abstract

The invention relates generally to sample ionization, and provides ionization probe assemblies, systems, computer program products, and methods useful for this purpose.

Claims

1. An ionization probe assembly, comprising:

at least one probe mounting structure;

at least one probe that is movably coupled to the probe mounting structure, which probe is configured to discontinuously introduce sample aliquots into an ion source housing; and

,

at least one probe conveyance mechanism operably connected to the probe, which probe conveyance mechanism is configured to convey the probe between at least a first position and at least a second position, wherein the first position is substantially electrically isolated from the second position.

2. The ionization probe assembly of claim 1, wherein the probe mounting structure comprises at least one view port.

3. The ionization probe assembly of claim 1, comprising at least one cover operably connected to the probe mounting structure.

4. An electrospray ion source housing comprising the ionization probe assembly of claim 1.

5. A mass spectrometer comprising the electrospray ion source housing of claim 4.

6. The ionization probe assembly of claim 1, wherein the probe mounting structure comprises an ion source housing back plate that is configured to operably connect to an ion source housing.

7. The ionization probe assembly of claim 6, wherein the ion source housing back plate comprises at least one alignment feature that is structured to align the ion source housing back plate relative to the ion source housing when the ion source housing back plate operably connects to the ion source housing.

8. The ionization probe assembly of claim 1, wherein at least one channel is disposed through a length of the probe.

9. The ionization probe assembly of claim 8, wherein the probe comprises at least one sprayer needle that fluidly communicates with the channel.

10. The ionization probe assembly of claim 8, wherein at least one nebulizer gas source and/or nebulizer gas sheath fluidly communicates with the channel.

11. The ionization probe assembly of claim 1, comprising at least one thermal modulator operably connected to the probe, which thermal modulator is configured to modulate a temperature of the probe.

12. The ionization probe assembly of claim 11, wherein the thermal modulator comprises a nebulizer gas heater.

13. The ionization probe assembly of claim 11, comprising at least one controller circuit board operably connected to the thermal modulator.

14. The ionization probe assembly of claim 1, comprising at least a first mounting component operably connected to the probe mounting structure, which first mounting component is configured to engage at least a second mounting component that is operably connected to an ion source housing when the probe mounting structure is mounted on the ion source housing.

15. The ionization probe assembly of claim 14, wherein the first and second mounting components comprise hinge and/or latch components.

16. The ionization probe assembly of claim 1, comprising at least one cavity disposed in or proximal to the probe mounting structure, which cavity comprises the second position.

17. The ionization probe assembly of claim 16, wherein the cavity fluidly communicates with at least one outlet.

18. The ionization probe assembly of claim 1, comprising at least two probes that are each movably coupled to the probe mounting structure.

19. The ionization probe assembly of claim 18, wherein the probes are independently movably coupled to the probe mounting structure.

20. The ionization probe assembly of claim 1, wherein the probe mounting structure comprises an ion source housing.

21. The ionization probe assembly of claim 20, wherein the ion source housing comprises at least one view port.

22. The ionization probe assembly of claim 1, comprising at least two probes independently movably coupled to the probe mounting structure.

23. The ionization probe assembly of claim 22, wherein each probe is movably coupled to the probe mounting structure via a pivot mechanism.

24. The ionization probe assembly of claim 23, wherein the probe conveyance mechanism comprises at least one motor operably connected to at least one of the pivot mechanisms via a pulley and belt drive assembly.

25. The ionization probe assembly of claim 22, wherein each probe is configured to move between a spray position and a rinse position, wherein the spray position is substantially electrically isolated from the rinse position.

26. The ionization probe assembly of claim 25, comprising at least one cavity disposed in or proximal to the probe mounting structure, which cavity comprises at least one of the rinse positions.

27. The ionization probe assembly of claim 26, wherein the cavity fluidly communicates with at least one outlet.

28. The ionization probe assembly of claim 1, wherein the probe is movably coupled to the probe mounting structure via a slide mechanism.

29. The ionization probe assembly of claim 28, wherein the slide mechanism comprises at least two probes.

30. The ionization probe assembly of claim 29, wherein the probes are substantially fixedly coupled to the slide mechanism.

31. The ionization probe assembly of claim 29, wherein the first position comprises a spray position and wherein the second position comprises at least first and second rinse positions that are each substantially electrically isolated from the spray position.

32. The ionization probe assembly of claim 31, wherein when a first probe is in the spray position, a second probe is in the second rinse position, and when the second probe is in the spray position, the first probe is in the first rinse position.

33. The ionization probe assembly of claim 28, wherein the slide mechanism comprises a probe support plate coupled to the probe mounting structure via a linear slide, and wherein the probe is mounted on the probe support plate.

34. The ionization probe assembly of claim 33, wherein the probe conveyance mechanism comprises a dual acting pneumatic cylinder operably connected to the probe mounting structure and to the probe support plate.

35. The ionization probe assembly of claim 1, wherein said at least one probe comprises at least one wide-bore probe.

36. The ionization probe assembly of claim 1, wherein said probe mounting structure comprises a removable cartridge.

37. The ionization probe assembly of claim 36, wherein said removable cartridge is spring-loaded.

38. The ionization probe assembly of claim 36, wherein said probe mounting structure is configured to accept removable cartridges from a variety of commercial instruments.

39. The ionization probe assembly of claim 1, further comprising one or more nebulizer gas lines configured to deliver gas from a nebulizer gas source to said at least one probe.

40. The ionization probe assembly of claim 39, wherein said one or more nebulizer gas lines comprise a thermal modulator to heat gas within said one or more nebulizer gas lines.