System and process for separating a material

• US 8,801,586 B2
• Filed: 12/20/2012
• Issued: 08/12/2014
• Est. Priority Date: 02/29/2008
• Status: Active Grant

• Current Assignee: Biomet Manufacturing, LLC (Zimmer Biomet Holdings, Inc.)
• Original Assignee: Biomet Biologics LLC (Zimmer Biomet Holdings, Inc.)
• Inventors: Dorian, Randel, Leach, Michael D., Storrs, Richard W., Chavarria, Jason
• Primary Examiner(s): Reifsnyder, David A
• Application Number:
  US13/721,978
• Publication Number:
  US 20130196425A1
• Time in Patent Office: 600 Days
• Field of Search: 210/120, 210/360.1, 210/380.1, 210/416.1, 422/72, 422/527, 494/36, 494/40, 494/43, 494/60, 604/403
• US Class Current: 494/40
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: B01L 3/5635 connecting two containers face to face, e.g. comprising a filter
• CPC Class Codes: C12M 47/02 Separating microorganisms from the culture medium; Concentration of biomass separating microorganisms from their culture media C12N1/02
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: G01N 1/286 involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising microtomes G01N1/06; pulverising in general B02C; mixing in general B01F
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids

Abstract

Disclosed is a system to separate, enrich, and/or purify a cellular population from a biological tissue, such as a tissue sample. For example, an adipose tissue sample can be acquired and disrupted. The disrupted tissue sample can then be separated and purified. The separated components can include multipotent, pluripotent, or other cell populations.

Claims

1. A system to separate a selected cell fraction from a sample volume, comprising:

a container having a first wall extending from a first bottom wall to an upper rim;

a collection section positioned near the upper rim and at an exterior surface of the first wall, the collection section having a second wall extending from a second bottom wall wherein the second bottom wall is nearer the upper rim than the first bottom wall;

a lid covering both the container and the collection section;

wherein the lid is operable to move between an open position and a closed position to selectively close the container from the collection section.

2. The system of claim 1, further comprising:

a drive motor operable to spin the container and the collection section simultaneously.

3. The system of claim 1, wherein the collection section at least directly contacts an exterior surface of the first wall.

4. The system of claim 3, wherein the upper rim of the container defines an annular section;

wherein the collection section is an annular well surrounding the annular section.

5. The system of claim 4, further comprising:

a sump defined within the collection section;

wherein the selected cell fraction is operable to collect within the sump defined within the collection section.

6. The system of claim 4, further comprising:

a barrier wall extending a width of the collection section;

wherein the barrier wall defines a first end and a second end of the collection section;

wherein a suction force can be applied at the first end to move the selected cell fraction within the collection section.

7. The system of claim 1, further comprising:

a vent defined through the collection section to allow venting of the collection section at least during withdrawal of the selected cell fraction from the collection section.

8. The system of claim 1, further comprising:

a disruption system operable to disrupt a volume to form sample particles as the sample volume.

9. The system of claim 8, wherein the disruption system includes a frit member positioned over an end of a sample holding container;

a forcing member operable to force the sample volume through the frit member to obtain the sample particle.

10. The system of claim 1, further comprising:

an exterior housing to house at least the container and the collection section;

a first port extending through the exterior housing to introduce the sample volume into the container; and

a second port extending through the exterior housing to allow access to the collection section to remove the selected cell fraction from the collection section.

11. A system to separate a selected cell fraction from a sample volume, comprising:

an outer housing;

an inner container housed within the outer housing, the inner container having a main container area and an isolation area spaced from the main container area with a wall portion therebetween;

a lid covering both the main container area and the isolation area; and

a sealing member extending from the wall portion between the main container area and the isolation area, wherein the lid is operable to move between an open position and a closed position to selectively close the main container area from the isolation area.

12. The system of claim 11, further comprising a drive base having a drive motor, the drive base being coupled to the outer housing and the inner container to rotate the inner container about a central axis.

13. The system of claim 11, wherein the isolation area is an annular well surrounding the main container area.

14. The system of claim 13, further comprising a sump defined within the isolation area, wherein the selected cell fraction is operable to collect within the sump defined within the isolation area.

15. The system of claim 14, further comprising a barrier wall extending a width of the isolation area;

wherein the barrier wall defines a first end and a second end of the isolation area;

wherein a suction force can be applied at a first end to move the selected cell fraction within the isolation area.

16. The system of claim 11, wherein the wall portion is an annular rim and the sealing member is an O-ring positioned in the wall portion.

17. The system of claim 11, further comprising:

a disruption system operable to disrupt a volume to form sample particles as the sample volume.

18. A system to separate a selected cell fraction from a sample volume, comprising:

a container having a main container area and an isolation area spaced from the main container area with a wall portion therebetween, the isolation area forming an annular well around the main container area having a sump defined within the annular well;

a barrier wall extending a width of the isolation area, where the barrier wall defines a first end and a second end of the isolation area;

a lid covering both the main container area and the isolation area; and

a sealing member extending from the wall portion between the main container area and the isolation area, wherein the lid is operable to move between an open position and a closed position to selectively close the main container area from the isolation area.

19. The system of claim 18, further comprising a port positioned relative to the sump, wherein the selected fraction is operable to collect within the sump defined within the isolation area and a suction force can be applied at the first end to move the selected fraction within the isolation area.

20. The system of claim 18, further comprising:

an exterior housing to house at least the container and the isolation area;

a first port extending through the exterior housing to introduce the sample volume into the container; and

a second port extending through the exterior housing to allow access to the isolation area to remove the selected cell fraction from the isolation area.