Biochip and manufacturing method thereof
First Claim
1. A method for manufacturing a biochip, comprising:
- coating a first self-assembled monolayer on a surface of a substrate;
forming a plurality of first biomedical molecular dots on the first self-assembled monolayer;
vaporously depositing a second self-assembled monolayer on the first self-assembled monolayer, wherein the second self-assembled monolayer and the first self-assembled monolayer are bound with each other;
wherein each of the first biomedical molecular dots includes a first portion of the first biomedical molecules bonded to the first self-assembled monolayer and a second portion of the first biomedical molecules masking the first portion of the first biomedical molecules to block the first portion of first biomedical molecules from contacting material forming the second self-assembled monolayer; and
removing the second portion of the first biomedical molecules and the second self-assembled monolayer adhered to the excess first biomedical molecules;
wherein the second self-assembled monolayer is a trichloro(1H,1H,2H,2H-perfluorooctyl)silane film, and trichloro(1H,1H,2H,2H-perfluorooctyl)silane has a molecular formula of CF3(CF2)5CH2CH2SiCl3.
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Abstract
A method for manufacturing a biochip is provided. First, a first self-assembled monolayer is coated on a substrate. Next, a plurality of first biomedical molecular dots are formed on the first self-assembled monolayer by micro-titration technique. After the bonding reaction between the first biomedical molecular point and the first self-assembled monolayer, a second self-assembled monolayer is deposited on the surface of the first self-assembled monolayer by evaporation. The second self-assembled monolayer attached on the plurality of first biomedical molecular dots and the first self-assembled monolayer not bonded to the substrate are removed, so that the first biomedical molecular dots immobilized on the first self-assembled monolayer are exposed. Finally, a second biomedical molecular layer is immobilized on the exposed portions of the first biomedical molecular dots.
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Citations
6 Claims
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1. A method for manufacturing a biochip, comprising:
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coating a first self-assembled monolayer on a surface of a substrate; forming a plurality of first biomedical molecular dots on the first self-assembled monolayer; vaporously depositing a second self-assembled monolayer on the first self-assembled monolayer, wherein the second self-assembled monolayer and the first self-assembled monolayer are bound with each other;
wherein each of the first biomedical molecular dots includes a first portion of the first biomedical molecules bonded to the first self-assembled monolayer and a second portion of the first biomedical molecules masking the first portion of the first biomedical molecules to block the first portion of first biomedical molecules from contacting material forming the second self-assembled monolayer; andremoving the second portion of the first biomedical molecules and the second self-assembled monolayer adhered to the excess first biomedical molecules; wherein the second self-assembled monolayer is a trichloro(1H,1H,2H,2H-perfluorooctyl)silane film, and trichloro(1H,1H,2H,2H-perfluorooctyl)silane has a molecular formula of CF3(CF2)5CH2CH2SiCl3. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification