Structures for controlling light interaction with microfluidic devices

• US 8,802,029 B2
• Filed: 05/20/2013
• Issued: 08/12/2014
• Est. Priority Date: 02/02/2009
• Status: Active Grant

• Current Assignee: Opko Diagnostics, LLC (Opko Health, Inc.)
• Original Assignee: Opko Diagnostics, LLC (Opko Health, Inc.)
• Inventors: Steinmiller, David, Linder, Vincent
• Primary Examiner(s): Sines, Brian J
• Application Number:
  US13/898,028
• Publication Number:
  US 20130252321A1
• Time in Patent Office: 449 Days
• Field of Search: 422/50, 422/68.1, 422/81, 422/82.05, 422/82.07, 422/82.08, 422/82.09, 422/82.11, 422/502, 422/503, 436/43, 436/164, 436/172, 356/73.1, 356/328, 356/656, 356/318
• US Class Current: 422/503
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/04 Closures and closing means
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: C12Y 304/21077 Semenogelase (3.4.21.77), i.e. prostate specific antigen or PSA or kallikrein 3
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/59 Transmissivity G01N21/25 takes precedence
• CPC Class Codes: G01N 2333/96455 Kallikrein (3.4.21.34; 3.4.21.35)
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: G01N 33/57488 involving compounds identifable in body fluids
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

Systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems.

Claims

1. A fluidic device comprising:

an article comprising first and second sides;

a first microfluidic channel segment positioned at the first side of the article;

first and second adjacent optical elements positioned at the second side of the article, wherein the first microfluidic channel segment is positioned substantially between the first and second optical elements,

wherein the first optical element is adapted and arranged such that when a portion of the article is exposed to light, the first optical element redirects at least a portion of the light away from a surface portion of the first side of the article, the surface portion being adjacent to the first microfluidic channel segment.

2. A fluidic device as in claim 1, wherein the first and/or second optical element is integral to a surface of the article.

3. A fluidic device as in claim 1, comprising a plurality of microfluidic channel segments.

4. A fluidic device as in claim 1, wherein the first optical element is adapted and arranged such that when the second side of the article is exposed to light at a first intensity at an angle of incidence between 80°

to 95°

, the surface portion of the first side of the article is not exposed to light or is exposed to the light at a second intensity lower than the intensity of the light at the surface portion absent the first optical element.

5. A fluidic device as in claim 1, wherein the first and/or second optical element has a substantially triangular cross-section.

6. A fluidic device as in claim 1, wherein the first and/or second optical element is concave.

7. A fluidic device as in claim 1, wherein the first and/or second optical element is convex.

8. A fluidic device as in claim 1, comprising a second microfluidic channel segment, wherein no line drawn between any first point on or within the first microfluidic channel segment and any second point on or within the second microfluidic channel segment intersects any point on or within the first optical element.

9. A fluidic device as in claim 1, wherein no line drawn between any first point on or within the first optical element and any second point on or within the second optical element intersects any point on or within the first microfluidic channel segment.

10. A fluidic device as in claim 1, wherein the first side is a first surface of the article.

11. A fluidic device as in claim 10, wherein the second side is a second surface of the article.

12. A fluidic device as in claim 1, wherein the first and/or second optical element comprises a cross-sectional dimension of at least about 50 microns and less than 1 cm.

13. A fluidic device as in claim 1, wherein the first and/or second optical element comprises a substantially opaque material.

14. A fluidic device as in claim 1, wherein the first and/or second optical element comprises a reflective surface.

15. A fluidic device as in claim 1, wherein the first and/or second optical element comprises an open channel.

16. A fluidic device as in claim 1, wherein the first and/or second optical element comprises a substantially enclosed channel.

17. A fluidic device as in claim 1, wherein the first and/or second optical element comprises a fluid.

18. A fluidic device as in claim 17, wherein the fluid comprises a dye.

19. A fluidic device as in claim 1, wherein the first and/or second optical element has a draft angle between about 12°

and about 60°

.

20. A fluidic device as in claim 1, wherein the first and/or second optical element has a draft angle between about 30°

and about 40°

.

21. A fluidic device as in claim 1, wherein the first microfluidic channel segment is part of a serpentine channel.

22. A fluidic device as in claim 1, wherein the article is a single, integral piece of material without joined layers.

23. A fluidic device as in claim 1, wherein the article is formed of a polymeric material, wherein the polymeric material is selected from polystyrene, cyclo-olefin-copolymer, polymethylmethacrylate, and polycarbonate.

24. A fluidic device as in claim 1, wherein the first and/or second optical element is substantially transparent to visible light.

25. A fluidic device as in claim 1, wherein the article is made by injection molding.

26. A fluidic device as in claim 1, wherein the first optical element is adapted and arranged to direct at least 50% of the light exposed to the first optical element away from the surface portion of the first side of the article.

27. A fluidic device as in claim 1, comprising a second microfluidic channel segment and an intervening portion positioned substantially between the first and second microfluidic channel segments, wherein the first optical element has a longest width that is greater than or equal to the width of the intervening portion, but less than the combination of the widths of the first and second microfluidic channel segments and the width of the intervening portion.

28. A fluidic device as in claim 1, further comprising a cover positioned over the first microfluidic channel segment to substantially enclose the first microfluidic channel segment.

29. A fluidic device as in claim 1, further comprising an analysis region in fluid communication with the first microfluidic channel segment.

30. A fluidic device as in claim 29, wherein the analysis region comprises a binding partner associated with a surface of the first microfluidic channel segment.

31. A fluidic device as in claim 30, wherein the binding partner comprises an antibody or an antigen.

32. A fluidic device as in claim 3, further comprising a detector associated with one or more microfluidic channel segments, wherein the detector is adapted and arranged to determine light transmission and/or absorbance through the one or more microfluidic channel segments.

33. A fluidic device as in claim 1, wherein the first optical element is adapted and arranged such that when a portion of the article is exposed to light at a first intensity, the first optical element redirects at least a portion of the light away from the surface portion of the first side of the article, such that the surface portion is exposed to the light at a second intensity at least 25% lower than an intensity of the light at the surface portion absent the first optical element.

34. A fluidic device as in claim 1, wherein the first optical element is adapted and arranged such that when a portion of the article is exposed to light at a first intensity, the first optical element redirects at least a portion of the light away from the surface portion of the first side of the article, such that the surface portion is exposed to the light at a second intensity at least 75% lower than an intensity of the light at the surface portion absent the first optical element.

35. A fluidic device as in claim 33, wherein the second intensity is at least 50% lower than the first intensity.

36. A fluidic device as in claim 1, comprising a second microfluidic channel segment, wherein the first and second microfluidic channel segments are sections of a microfluidic channel comprising a meandering configuration including multiple turns, each turn of the meandering channel being a different microfluidic channel segment.

37. A fluidic device as in claim 36, wherein the first and/or second microfluidic channel segment has a length of at least 1 cm.