Method for treating textile with endoglucanase

• US 8,802,423 B2
• Filed: 12/14/2011
• Issued: 08/12/2014
• Est. Priority Date: 12/30/2010
• Status: Expired due to Fees

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Lai, Weijian, Wu, Guifang, Liu, Ye, Zhou, Yucheng, Han, Yang
• Primary Examiner(s): Prouty, Rebecca
• Assistant Examiner(s): EKSTROM, RICHARD C
• Application Number:
  US13/997,774
• Publication Number:
  US 20130295651A1
• Time in Patent Office: 972 Days
• Field of Search: None
• US Class Current: 435/263
• CPC Class Codes: C11D 3/38645 containing cellulase
• CPC Class Codes: D06M 16/003 with enzymes or microorganisms
• CPC Class Codes: D06P 1/228 Indigo
• CPC Class Codes: D06P 5/137 with other compounds
• CPC Class Codes: D06P 5/158 with other compounds

Abstract

The present invention relates to the method for manufacturing textile, by treating textile with an isolated polypeptide having endoglucanase activity, especially in biostoning and bio-polishing process.

Claims

1. A method for treating a textile, comprising treating the textile with a polypeptide having endoglucanase activity, wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 22-237 of SEQ ID NO:

2 and does not comprise a functional cellulose binding domain.

2. The method of claim 1, wherein the polypeptide has at least 97% sequence identity to the sequence of amino acids 22-237 of SEQ ID NO:

2.

3. The method of claim 1, wherein the polypeptide has at least 99% sequence identity to the sequence of amino acids 22-237 of SEQ ID NO:

2.

4. The method of claim 1, wherein the polypeptide comprises the sequence of amino acids 22-237 of SEQ ID NO:

2.

5. The method of claim 1, wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:

1, or (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO;

1, or (iii) the full-length complementary strand of (i) or (ii).

6. The method of claim 1, wherein the polypeptide is a variant of the polypeptide of SEQ ID NO:

2, which comprises a substitution, deletion and/or insertion at one or more positions.

7. The method of claim 6, wherein the polypeptide contains a histidine residue at a position corresponding to position 141 of SEQ ID NO:

2.

8. The method of claim 1, wherein the method is a biostoning process resulting in localized variation of color density in the surface the textile.

9. The method of claim 8, wherein the textile is dyed cellulosic or cellulose-containing fabric.

10. The method of claim 8, wherein the biostoning process achieves an abrasion level of at least 0.5 Delta L* unit.

11. The method of claim 8, wherein the biostoning process achieves a backstaining level of at least 0.1 Delta b* unit increase.

12. The method of claim 1, wherein the method is applied in a biopolishing process.

13. The method of claim 12, wherein the biopolishing process results in a pilling note of at least 3.

14. The method of claim 12, wherein the biopolishing process shows a weight loss of less than 10%.

15. The method of claim 1, wherein the method is conducted at a temperature below 90°

C.

16. The method of claim 15, wherein the method is conducted at a temperature below 65°

C.

17. The method of claim 16, wherein the method is conducted at a temperature below 40°

C.

18. The method of claim 1, wherein the method is conducted at a pH in the range of 4.5 to 7.5.

19. The method of claim 1, wherein the polypeptide is applied in an amount in the range of 0.05-2 milligram of enzyme protein per gram of textile.

20. The method of claim 1, which further comprises treating the textile with one or more enzymes selected from the group consisting of proteases, lipases, cutinases, amylases, pectinases, hemicellulases and cellulases.