Articles having localized molecules disposed thereon and methods of producing same

• US 8,802,600 B2
• Filed: 03/29/2007
• Issued: 08/12/2014
• Est. Priority Date: 03/30/2006
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Rank, David R., Wegener, Jeffery, Korlach, Jonas, Roitman, Daniel, Xu, Yue, Lyle, John, Turner, Stephen, Peluso, Paul, Otto, Geoff, Cicero, Ron
• Primary Examiner(s): Gross, Christopher M
• Application Number:
  US11/731,748
• Publication Number:
  US 20080032301A1
• Time in Patent Office: 2,693 Days
• Field of Search: None
• US Class Current: 506/43
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00427 using masks
• CPC Class Codes: B01J 2219/0045 using optical fibres
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00639 the compounds being trapped in or bound to a porous medium
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B82Y 20/00 Nanooptics, e.g. quantum optics or photonic crystals
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2527/127 the enzyme inhibitor or activator used
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: Y10T 29/49002 : Electrical device making

Abstract

Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Claims

1. A zero mode waveguide array, comprising:

a plurality of zero mode waveguide cores disposed in a cladding layer, each core having a bottom reading surface;

wherein a plurality of zero mode waveguide cores in the array comprise an enzyme bound to the bottom surface;

wherein portions of the array other than the bottom surface of the cores are substantially free of the enzyme; and

wherein the cladding layer comprises a compound comprising one or more phosphonic acid groups.

2. The zero mode waveguide array of claim 1, wherein the cladding layer is disposed on a silica based substrate.

3. The zero mode waveguide array of claim 1, wherein the cladding comprises a metal or metal oxide.

4. The zero mode waveguide array of claim 1, wherein the plurality of zero mode waveguide cores in the array have only a single active enzyme molecule bound to the bottom surface.

5. The zero mode waveguide array of claim 4, wherein the enzyme comprises a polymerase enzyme.

6. The zero mode waveguide array of claim 1, wherein the enzyme is bound to the bottom surface through one or more silanes.

7. The zero mode waveguide array of claim 1, wherein the enzyme is bound to the bottom surface through a binding or coupling group.

8. The zero mode waveguide array of claim 7, wherein the binding or coupling group comprises an antibody, an antibody fragment, avidin, biotin, a binding peptide, a lectin, or complementary nucleic acids.

9. The zero mode waveguide array of claim 1, wherein the enzyme is bound to the bottom surface through biotin.

10. The zero mode waveguide array of claim 1, wherein the enzyme is bound to the bottom surface through a phospholipid bilayer.

11. The zero mode waveguide array of claim 9, wherein the enzyme is bound to the bottom surface through a biotin-PEG-silane.

12. The method of claim 1, wherein the compound comprising one or more phosphonic acid groups is polyvinylphosphonic acid.

13. The method of claim 1, wherein the compound comprising one or more phosphonic acid groups is selected from the group consisting of:

14. The method of claim 1, wherein the compound comprising one or more phosphonic acid groups is selected from the group consisting of:

2-carboxyethyl phosphonic acid;

amino tri (methylene phosphonic acid);

1-hydroxyethylidene -1,1,-diphosphonic acid;

hexamethylenediaminetetra (methylenephosphonic acid);

diethylenetriamine penta(methylene phosphonic acid);

ethylenediamine tetra(methylene phosphonic acid);

bis(hexamethylene triamine penta(methylenephosphonic acid));

2-phosphonobutane - 1,2,4 - tricarboxylic acid; and

monoethanloamine diphosphonate.

15. The method of claim 1, wherein the compound comprising one or more phosphonic acid groups comprises an alkyl phosphonate.

16. The method of claim 1, wherein the compound comprising one or more phosphonic acid groups is selected from the group consisting of:

octyl phosphonic acid, decyl phosphonic acid, dodecyl phosphonic acid, hexadecyl phosphonic acid, octadecyl phosphonic acid, docosyl phosphonic acid, hydroxy-dodecyl phosphonic acid, hydroxy-undecenyl-phosphonic acid, and decanediylbis(phosphonic acid).