Materials and methods for prevention and treatment of RNA viral diseases
First Claim
1. A method of increasing expression of 2′
- -5′
oligoadenylate synthetase in a mammal, said method comprising administering to the mammal a nucleotide sequence encoding a 2′
-5′
oligoadenylate synthetase, or at least one enzymatically active fragment thereof, wherein the nucleotide sequence is expressed in the mammal.
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Abstract
The subject invention concerns a method of inhibiting an RNA virus infection within a patient by increasing the amount of 2-5 oligoadenylate synthetase (2-5 AS) activity within the patient. Preferably, the preventative and therapeutic methods of the present invention involve administering a nucleotide encoding 2-5 AS, or at least one catalytically active fragment thereof, such as the p40, p69, p100 subunits, to a patient in need thereof. The present inventors have determined that overexpression of 2-5AS causes a reduction in epithelial cell damage, reduction in infiltration of mononuclear cells in the peribronchiolar and perivascular regions, and reduction in thickening of the septa in the lungs. Levels of chemokines, such as MIP1-α, are also reduced upon overexpression of 2-5AS. The subject invention also pertains to pharmaceutical compositions containing a nucleotide sequence encoding 2-5 AS and a pharmaceutically acceptable carrier, as well as vectors for delivery of the 2-5 AS nucleotide sequence.
56 Citations
8 Claims
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1. A method of increasing expression of 2′
- -5′
oligoadenylate synthetase in a mammal, said method comprising administering to the mammal a nucleotide sequence encoding a 2′
-5′
oligoadenylate synthetase, or at least one enzymatically active fragment thereof, wherein the nucleotide sequence is expressed in the mammal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- -5′
Specification