Systems and methods for performing optical spectroscopy using a self-calibrating fiber optic probe

• US 8,804,115 B2
• Filed: 04/27/2009
• Issued: 08/12/2014
• Est. Priority Date: 04/25/2008
• Status: Active Grant

• Current Assignee: Duke University
• Original Assignee: Duke University
• Inventors: Yu, Bing, Ramanujam, Nirmala
• Primary Examiner(s): Toatley, Gregory J
• Assistant Examiner(s): SMITH, MAURICE C
• Application Number:
  US12/989,591
• Publication Number:
  US 20110295541A1
• Time in Patent Office: 1,933 Days
• Field of Search: 356330-334, 356400-425, 356/456
• US Class Current: 356/300
• CPC Class Codes: G01J 2003/2866 Markers; Calibrating of scan
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/28 Investigating the spectrum using colour filters G01J3/51
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/474 Details of optical heads therefor, e.g. using optical fibres
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 2201/082 Fibres for a reference path

Abstract

Systems and methods for performing optical spectroscopy using a self-calibrating fiber optic probe are disclosed. One self-calibrating fiber optic probe includes a sensing channel for transmitting illumination light to a specimen and for collecting spectral data of the specimen. The spectral data includes the illumination light diffusely reflected from the specimen at one or more wavelengths. The self-calibrating fiber optic probe may also include a calibration channel for transmitting calibration light. The calibration light and the illumination light are generated simultaneously from a common light source. The calibration channel collects calibration spectral data associated with the calibration light contemporaneously with the collection of the spectral data of the specimen.

Claims

1. A self-calibrating fiber optic probe comprising:

a sensing channel for transmitting illumination light to a specimen and for collecting spectral data of the specimen, wherein the spectral data includes the illumination light diffusely reflected from the specimen at one or more wavelengths, wherein the sensing channel includes at least one illumination source fiber for transmitting the illumination light to the specimen and at least one detection fiber for collecting the illumination light diffusely reflected from the specimen; and

a calibration channel for transmitting calibration light, wherein the calibration light and the illumination light are generated simultaneously from a common light source, and collecting calibration spectral data associated with the calibration light contemporaneously with the collection of the spectral data of the specimen, wherein the calibration channel includes at least one calibration source fiber for transmitting the calibration light and at least one calibration return fiber for receiving at least a portion of the calibration light from the at least one calibration source fiber and wherein the at least one calibration source fiber and the at least one illumination source fiber comprise separate fibers having identical numerical apertures.

2. The fiber optic probe of claim 1 wherein the at least one calibration source fiber and the at least one calibration return fiber are the same optical fiber.

3. The fiber optic probe of claim 2 wherein the at least one calibration source fiber is bent within a probe tip housing section in the fiber optic probe such that the at least one calibration source fiber functions as the at least one calibration return fiber.

4. The fiber optic probe of claim 1 wherein the at least one calibration source fiber and the at least one illumination source fiber are constructed from the same type of materials and include identical core diameters and clad diameters, and wherein the at least one detection fiber and the at least one calibration return fiber are constructed from the same type of materials and include identical numerical apertures, core diameters and clad diameters.

5. The fiber optic probe of claim 1 wherein the at least one calibration source fiber directs at least a portion of the calibration light to a reflective element to be reflected to and received by the at least one calibration return fiber.

6. The fiber optic probe of claim 5 wherein the reflective element includes at least one of a mirror, a reflective rod, and a polished metal element.

7. The fiber optic probe of claim 1 wherein the at least one calibration return fiber and the at least one detection fiber are coupled to at least one of a spectrograph, spectrometer, or photodetector.

8. The fiber optic probe of claim 1 wherein the at least one calibration return fiber is coupled to at least one of a first spectrograph, a first spectrometer, or a first photodetector, and the at least one detection fiber is coupled to at least one of a second spectrograph, a second spectrometer, or a second photodetector.

9. The fiber optic probe of claim 1 wherein the at least one calibration return fiber includes a primary calibration return fiber and a backup calibration return fiber.

10. The fiber optic probe of claim 1 wherein the specimen includes a tissue or a turbid medium.

11. The fiber optic probe of claim 1 wherein the fiber optic probe comprises at least one of a forward firing fiber optic probe or a side firing fiber optic probe.

12. The fiber optic probe of claim 1 wherein the calibration channel includes a reflective element, wherein the at least one calibration source fiber directs at least a portion of the calibration light to the reflective element to be reflected to and received by the at least one calibration return fiber, wherein the calibration channel further includes a housing for housing the at least one calibration source fiber, the at least one calibration return fiber, and the reflective element, wherein the at least one calibration source fiber terminates within the housing, and wherein the at least one illumination source fiber extends completely through the housing to interface with the specimen.

13. A system for performing self-calibrating diffuse reflectance spectroscopy, the system comprising:

a fiber optic probe that includes;

a sensing channel for transmitting illumination light to a specimen and for collecting spectral data of the specimen, wherein the spectral data includes the illumination light diffusely reflected at one or more wavelengths from the specimen, wherein the sensing channel includes at least one illumination source fiber for transmitting the illumination light to the specimen and at least one detection fiber for collecting the illumination light diffusely reflected from the specimen; and

a calibration channel for transmitting calibration light, wherein the calibration light and the illumination light are generated simultaneously from a light source, and for collecting calibration spectral data associated with the calibration light contemporaneously with the collection of the spectral data of the specimen, wherein the calibration channel includes at least one calibration source fiber for transmitting the calibration light and at least one calibration return fiber for receiving at least a portion of the calibration light from the at least one calibration source fiber and wherein the at least one calibration source fiber and the at least one illumination source fiber comprise separate fibers having identical numerical apertures;

a processing unit, coupled to the sensing channel and the calibration channel, for receiving the spectral data of the specimen and the calibration spectral data to generate calibrated specimen spectral data in real-time.

14. The system of claim 13 wherein the at least one calibration source fiber and the at least one calibration return fiber are the same optical fiber.

15. The system of claim 13 wherein the at least one calibration source fiber is bent within a probe tip housing section in the fiber optic probe such that the at least one calibration source fiber functions as the at least one calibration return fiber.

16. The system of claim 13 wherein the at least one calibration source fiber and the at least one illumination source fiber are constructed from the same type of materials and include identical, core diameters and clad diameters, and wherein the at least one detection fiber and the at least one calibration return fiber are constructed from the same type of materials and include identical numerical apertures, core diameters and clad diameters.

17. The system of claim 13 wherein the at least one calibration source fiber directs at least a portion of the calibration light to a reflective element to be reflected to and received by the at least one calibration return fiber.

18. The system of claim 17 wherein the reflective element includes at least one of a mirror, a reflective rod, and a polished metal element.

19. The system of claim 13 wherein the at least one calibration return fiber includes a primary calibration return fiber and a backup calibration return fiber.

20. The system of claim 13 wherein the specimen includes a tissue or a turbid medium.

21. The system of claim 13 wherein the fiber optic probe comprises at least one of a forward firing fiber optic probe or a side firing fiber optic probe.

22. The system of claim 13 wherein the light source includes at least one of a xenon lamp or a white light emitting diode (LED) source.

23. The system of claim 13, wherein the processing unit comprises a spectrometer and a computer for receiving contemporaneously the spectral data of the specimen from the at least one detection fiber and the calibration spectral data from the at least one calibration return fiber.

24. The system of claim 23 wherein the calibration spectral data represents effects of at least one of real-time light source intensity fluctuations and real-time attenuation of the calibration light caused by bending of the fiber optic probe.

25. The system of claim 24 wherein the processing unit applies a correlation factor to the calibration spectral data to generate modified calibration spectral data.

26. The system of claim 25 wherein the correlation factor comprises a ratio between spectral data associated with a diffuse reflectance standard and the calibration spectral data that is collected concurrently.

27. The system of claim 26 wherein the processing unit processes the spectral data of the specimen and the modified calibration spectral data to produce calibrated specimen spectral data.

28. The system of claim 27 wherein the calibrated specimen spectral data is an input to a Monte Carlo algorithm or a diffusion algorithm that derives optical properties of the specimen.

29. The system of claim 28 wherein the Monte Carlo algorithm includes either an inverse Monte Carlo reflectance algorithm or an inverse Monte Carlo fluorescence algorithm.

30. The system of claim 29 wherein the diffusion algorithm includes an inverse diffusion algorithm.

31. The system of claim 13 wherein the calibration channel includes a reflective element, wherein the at least one calibration source fiber directs at least a portion of the calibration light to the reflective element to be reflected to and received by the at least one calibration return fiber, wherein the calibration channel further includes a housing for housing the at least one calibration source fiber, the at least one calibration return fiber, and the reflective element, wherein the at least one calibration source fiber terminates within the housing, and wherein the at least one illumination source fiber extends completely through the housing to interface with the specimen.

32. A method for utilizing a self-calibrating fiber optic probe comprising:

transmitting illumination light via a sensing channel from a light source to a specimen, wherein the sensing channel includes at least one illumination source fiber for transmitting the illumination light to the specimen and at least one detection fiber for collecting the illumination light diffusely reflected from the specimen;

transmitting calibration light via a calibration channel, wherein the calibration light and the illumination light are generated simultaneously from the light source, wherein the calibration channel includes at least one calibration source fiber for transmitting the calibration light and at least one calibration return fiber for receiving at least a portion of the calibration light from the at least one calibration source fiber and wherein the at least one calibration source fiber and the at least one illumination source fiber comprise separate fibers having identical numerical apertures;

collecting spectral data of the specimen via the sensing channel, wherein the spectral data includes the illumination light diffusely reflected from the specimen at one or more wavelengths; and

collecting calibration spectral data associated with the calibration light via the calibration channel contemporaneously with the collection of the spectral data of the specimen.

33. The method of claim 32 wherein the at least one calibration source fiber and the at least one calibration return fiber are the same optical fiber.

34. The method of claim 33 wherein the at least one calibration source fiber is bent within a probe tip housing section in the method such that the at least one calibration source fiber functions as the at least one calibration return fiber.

35. The method of claim 32 wherein the at least one calibration source fiber and the at least one illumination source fiber are constructed from the same type of materials and include identical, core diameters and clad diameters, and wherein the at least one detection fiber and the at least one calibration return fiber are constructed from the same type of materials and include identical numerical apertures, core diameters and clad diameters.

36. The method of claim 32 further comprising:

directing at least a portion of the calibration light via the at least one calibration source fiber to a reflective element to be reflected to and received by the at least one calibration return fiber.

37. The method of claim 36 wherein the reflective element includes at least one of a mirror, a reflective rod, and a polished metal element.

38. The method of claim 32 wherein the at least one calibration return fiber and the at least one detection fiber are coupled to at least one of a spectrograph, spectrometer, or photodiode.

39. The method of claim 32 wherein the at least one calibration return fiber is coupled to at least one of a first spectrograph, a first spectrometer, or a first photodiode, and the at least one detector fiber is coupled to at least one of a second spectrograph, a second spectrometer, or a second photodiode.

40. The method of claim 32 wherein the at least one calibration return fiber includes a primary calibration return fiber and a backup calibration return fiber.

41. The method of claim 32 wherein the specimen includes a tissue or a turbid medium.

42. The method of claim 32 wherein the method comprises at least one of a forward firing fiber optic probe or a side firing fiber optic probe.

43. The method of claim 32 wherein the calibration channel includes a reflective element, wherein the at least one calibration source fiber directs at least a portion of the calibration light to the reflective element to be reflected to and received by the at least one calibration return fiber, wherein the calibration channel further includes a housing for housing the at least one calibration source fiber, the at least one calibration return fiber, and the reflective element, wherein the at least one calibration source fiber terminates within the housing, and wherein the at least one illumination source fiber extends completely through the housing to interface with the specimen.