Microfluidic device

• US 8,808,519 B2
• Filed: 12/14/2011
• Issued: 08/19/2014
• Est. Priority Date: 12/14/2011
• Status: Active Grant

• Current Assignee: Hamilton Sundstrand Space Systems International Inc (Rtx Corporation)
• Original Assignee: Hamilton Sundstrand Space Systems International Inc (Rtx Corporation)
• Inventors: Trubey, Richard K.
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US13/325,435
• Publication Number:
  US 20130153416A1
• Time in Patent Office: 979 Days
• Field of Search: 204/450, 204/451, 204/600, 204/601, 204/411
• US Class Current: 204/600
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements

Abstract

A microfluidic device includes a micro-channel that defines a plane. A plurality of drive electrodes is located on a first side of the plane. At least one ground electrode is operable to establish an electric potential with the plurality of drive electrodes. An array of electrochemical biosensors is located on a second, opposite side of the plane.

Claims

1. A microfluidic device comprising:

a micro-channel defining a plane;

a plurality of drive electrodes located on a first side of the plane, the drive electrodes being arranged in side-by-side rows;

at least one ground electrode operable to establish an electric potential with the plurality of drive electrodes; and

an array of electrochemical biosensors located on a second, opposite side of the plane, the electrochemical biosensors being arranged in side-by-side rows.

2. The microfluidic device as recited in claim 1, wherein the micro-channel extends between a first hydrophobic surface on the first side and a second hydrophobic surface on the second side.

3. The microfluidic device as recited in claim 1, wherein each electrochemical biosensor and each of the plurality of drive electrode defines a respective footprint area, and the footprint area of each of the electrochemical biosensors is less than the footprint area of each of the plurality of drive electrodes.

4. The microfluidic device as recited in claim 1, including a spacing distance between immediately neighboring ones of the electrochemical biosensors of 0.8-1.65 millimeters.

5. The microfluidic device as recited in claim 1, wherein each electrochemical biosensor is centered over a respective one of the plurality of drive electrodes.

6. The microfluidic device as recited in claim 1, including a sorbent material located within the micro-channel, the sorbent material being actively sorbent with regard to moisture.

7. The microfluidic device as recited in claim 1, wherein the side-by-side rows of electrochemical biosensors are parallel to each other and the side-by-side rows of drive electrodes are also parallel to each other.

8. The microfluidic device as recited in claim 1, wherein the electrochemical biosensors that are arranged in the side-by-side rows of electrochemical biosensors have a one-for-one correspondence to the drive electrodes that are arranged in the side-by-side rows of drive electrodes.

9. The microfluidic device as recited in claim 1, wherein a scalable ratio of a footprint area of one of the electrochemical biosensors to a footprint area of one of the drive electrodes is 1:

9 or less.

10. The microfluidic device as recited in claim 1, wherein the electrochemical biosensors of one of the side-by-side rows of electrochemical biosensors are configured to progressively identify a target analyte.

11. The microfluidic device as recited in claim 1, wherein one of the side-by-side rows of electrochemical biosensors includes a first electrochemical biosensor configured to detect a general class of target analyte and subsequent electrochemical biosensors are configured to detect, respectively, specific types of the general class of target analyte.

12. The microfluidic device as recited in claim 1, wherein the side-by-side rows of electrochemical biosensors are configured, by row, to detect different classes of target analytes.

13. A microfluidic device comprising:

a micro-channel defining a plane;

a plurality of drive electrodes located on a first side of the plane;

at least one ground electrode operable to establish an electric potential with the plurality of drive electrodes; and

an array of electrochemical biosensors located on a second, opposite side of the plane, wherein the micro-channel extends between a first hydrophobic surface on the first side and a second hydrophobic surface on the second side, and wherein the second hydrophobic surface includes a plurality of openings each corresponding in location to a respective one of the electrochemical biosensors such that each of the openings establishes fluid communication between its respective electrochemical biosensor and the micro-channel.

14. The microfluidic device as recited in claim 13, wherein each of the electrochemical biosensors includes a different immobilized biological material.

15. The microfluidic device as recited in claim 14, wherein the immobilized biological materials include at least one of antigens or antibodies.

16. The microfluidic device as recited in claim 14, wherein the immobilized biological materials include enzymes.

17. The microfluidic device as recited in claim 16, wherein the immobilized enzymes include an oxidoreductase enzyme.

18. The microfluidic device as recited in claim 13, including a first spacing distance D₁ between immediately neighboring ones of the electrochemical biosensors and a second spacing distance D₂ between immediately neighboring ones of the drive electrodes such that a scalable ratio of D₁:

D₂ is from 4;

1 to 33;

1.

19. The microfluidic device as recited in claim 18, wherein the scalable ratio is from 6:

1 to 18;

1.

20. The microfluidic device as recited in claim 18, wherein the scalable ratio is from 9:

1 to 13;

1.

21. A microfluidic device comprising:

a first plate; and

a second plate spaced apart from the first plate such that there is a micro-channel between the first plate and the second plate,

the first plate including a plurality of drive electrodes,

the second plate including an array of electrochemical biosensors, and

one of the first plate or the second plate includes at least one ground electrode operable to establish an electric potential with the plurality of drive electrodes, including a first spacing distance D₁ between immediately neighboring ones of the electrochemical biosensors and a second spacing distance D₂ between immediately neighboring ones of the plurality of drive electrodes such that a scalable ratio of D₁;

D₂ is from 4;

1 to 33;

1.

22. The microfluidic device as recited in claim 21, wherein the scalable ratio is from 6:

1 to 18;

1.

23. The microfluidic device as recited in claim 21, including a gasket extending around the perimeter of the micro-channel between the first plate and the second plate, at least one sample port opening extending through the first plate or the second plate, and at least one of the first plate and the second plate includes a recessed well.

24. The microfluidic device as recited in claim 21, wherein the at least one ground electrode includes a grid of wires defining spaces between the wires.

25. A microfluidic device comprising:

a first plate; and

a second plate spaced apart from the first plate such that there is a micro-channel between the first plate and the second plate,

the first plate including a plurality of drive electrodes,

the second plate including an array of electrochemical biosensors, and

one of the first plate and the second plate includes at least one ground electrode operable to establish an electric potential with the plurality of drive electrodes, wherein the at least one ground electrode includes a grid of wires defining spaces between the wires, and wherein each of the electrochemical biosensors is located between the wires in one of the spaces.