Small volume in vitro analyte sensor
First Claim
1. A method for determining a concentration of an analyte in a sample, comprising the steps of:
- (a) transporting a sample into a sample chamber of a sensor, the sample chamber comprising;
(i) an electrode pair comprising a working electrode disposed on a first substrate and a counter electrode disposed on a second substrate, wherein the working and counter electrodes are in a facing configuration;
(ii) a dielectric layer disposed between the working electrode and the counter electrode;
(iii) a sample chamber bounded in part by side walls of the dielectric layer, the working electrode and the counter electrode and,(iv) a sensing layer comprising an analyte-responsive enzyme and a redox mediator, wherein the sensing layer is disposed in the sample chamber and on the working electrode and at least a portion of the side walls of the dielectric layer;
(b) generating a sensor signal at the working electrode; and
(c) determining by coulometry the concentration of the analyte using the sensor signal.
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Accused Products
Abstract
A sensor designed to determine the concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a redox mediator that acts as an electron transfer agent between the analyte and the electrode. A second electron transfer agent, such as an enzyme, can be added to facilitate the electrooxidation or electroreduction of the analyte. Various electrochemical detection methods, such as amperometric, voltammetric, and potentiometric techniques, can be used to determine the analyte concentration. The sensor can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum.
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Citations
24 Claims
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1. A method for determining a concentration of an analyte in a sample, comprising the steps of:
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(a) transporting a sample into a sample chamber of a sensor, the sample chamber comprising; (i) an electrode pair comprising a working electrode disposed on a first substrate and a counter electrode disposed on a second substrate, wherein the working and counter electrodes are in a facing configuration; (ii) a dielectric layer disposed between the working electrode and the counter electrode; (iii) a sample chamber bounded in part by side walls of the dielectric layer, the working electrode and the counter electrode and, (iv) a sensing layer comprising an analyte-responsive enzyme and a redox mediator, wherein the sensing layer is disposed in the sample chamber and on the working electrode and at least a portion of the side walls of the dielectric layer; (b) generating a sensor signal at the working electrode; and (c) determining by coulometry the concentration of the analyte using the sensor signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification