Apparatus and method for separating and concentrating fluids containing multiple components

• US 8,808,551 B2
• Filed: 11/15/2010
• Issued: 08/19/2014
• Est. Priority Date: 05/24/2002
• Status: Active Grant

• Current Assignee: Biomet Manufacturing, LLC (Zimmer Biomet Holdings, Inc.)
• Original Assignee: Biomet Biologics LLC (Zimmer Biomet Holdings, Inc.)
• Inventors: Leach, Michael D., Higgins, Joel C., Swift, Matthew, Gordon, Nathan
• Primary Examiner(s): Mellon, David C
• Application Number:
  US12/946,338
• Publication Number:
  US 20110056893A1
• Time in Patent Office: 1,373 Days
• Field of Search: 210780-782, 210/787, 210/800, 210/109, 210/789, 210/360.1, 210/380.1, 210/369, 210/377, 210/382, 422/527, 422/533, 422/534, 422/535, 422/536, 422/548, 422/549, 422/547, 422/559, 436/177, 604/5.01, 604/6.01, 604/6.09, 604/6.15, 604/406, 494/36, 494/37, 494 16- 19, 494/32
• US Class Current: 210/789
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61M 1/3693 using separation based on different densities of components, e.g. centrifuging
• CPC Class Codes: B01L 1/52 Transportable laboratories; Field kits
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0605 check valves
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50215 using a float to separate phases
• CPC Class Codes: B01L 9/54 Supports specially adapted for pipettes and burettes automated pipetting stations G01N35/10
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence

Abstract

An apparatus that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at least three fractions in the apparatus. It also provides for a new method of extracting the buffy coat phase from a whole blood sample and mesenchymal stem cells from bone reaming material. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient.

Claims

1. A method of separating a fraction from a whole sample, comprising:

placing an obtained volume of a whole heterogeneous material, including at least a first liquid portion and a solid portion, obtained substantially simultaneously while reaming a bone, in a container having at least a first section and a second section;

separating the solid portion from the first liquid portion at least by retaining at least a first portion of the solid portion of the obtained volume of a whole heterogeneous material in the first section of the container and allowing a separated portion of the first liquid portion to move to the second section;

forming a fraction of at least the separated portion of the first liquid portion including at least undifferentiated cells in the second section of the container; and

extracting the formed fraction from the second section of the container at least by allowing a first member to contact a second member;

wherein forming the fraction of the liquid including at least undifferentiated cells in the second section of the container includes;

positioning at least the container including the second section in a centrifuge device; and

centrifuging the container in the centrifuge device so that a buoy including the first member and the second member moves within the second section;

wherein extracting the formed fraction from the second section of the container includes accessing a volume between the first member of the buoy and the second member of the buoy through a passage in the first member of the buoy;

wherein during extraction a first surface of the first member is configured to mate with a second surface of the second member.

2. The method of claim 1, wherein forming the fraction includes forming the fraction after the at least the separated portion of the first liquid portion has moved from the first section to the second section.

3. The method of claim 1, wherein separating the solid portion from the first liquid portion includes:

centrifuging the container including the placed volume of the whole heterogeneous material;

wherein at least a portion of the solid portion is supported on a filter in the first section of the container during the centrifuging and at least a portion of the first liquid portion moves to the second section of the container through the filter.

4. The method of claim 3, wherein a connection between the first section and the second section includes a cross-sectional dimension substantially similar to at least one of the first section or the second section.

5. The method of claim 1, wherein forming the fraction of the liquid including at least undifferentiated cells in the second section of the container includes:

positioning at least the container including the second section in a centrifuge device; and

centrifuging the container in the centrifuge device so that the buoy including the first member moves within the second section;

wherein extracting the formed fraction from the second section of the container includes accessing a volume between a plunger that includes the second member and the buoy through a passage in the plunger;

wherein during extraction a first surface of the plunger is configured to mate with a second surface of the buoy.