Method for rapid detection and identification of bioagents in epidemiological and forensic investigations
First Claim
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1. A method of identifying multiple different etiologic bioagents of a disease or condition, comprising:
- a) contacting nucleic acid from said multiple different etiologic bioagents of a disease or condition with two or more oligonucleotide primers that hybridize to sequence regions of said nucleic acid from said multiple different etiologic bioagents of a disease or condition that are conserved among different bioagents, wherein said conserved sequence regions flank variable sequence regions to produce two or more amplification products;
b) determining the base compositions of said two or more amplification products; and
c) identifying said multiple different etiologic bioagents of a disease or condition by comparing said determined base compositions to a database of base compositions comprising nucleic acid numbers but not nucleic acid sequences from a plurality of different bioagents.
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Abstract
The present invention provides methods for rapid forensic investigations by identification of bioagents associated with biowarfare and acts of terrorism or crime. The methods are also useful for epidemiological investigations by genotyping of bioagents.
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Citations
94 Claims
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1. A method of identifying multiple different etiologic bioagents of a disease or condition, comprising:
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a) contacting nucleic acid from said multiple different etiologic bioagents of a disease or condition with two or more oligonucleotide primers that hybridize to sequence regions of said nucleic acid from said multiple different etiologic bioagents of a disease or condition that are conserved among different bioagents, wherein said conserved sequence regions flank variable sequence regions to produce two or more amplification products; b) determining the base compositions of said two or more amplification products; and c) identifying said multiple different etiologic bioagents of a disease or condition by comparing said determined base compositions to a database of base compositions comprising nucleic acid numbers but not nucleic acid sequences from a plurality of different bioagents. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69)
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70. A system, comprising:
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a) a nucleic acid amplification component; b) a base composition determination component; c) a base composition identification component comprising a database of base compositions comprising nucleic acid numbers but not nucleic acid sequences from a plurality of bioagents wherein said bioagents comprise multiple different etiologic bioagents of a disease or condition. - View Dependent Claims (71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94)
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Specification