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Method for rapid detection and identification of bioagents in epidemiological and forensic investigations

  • US 8,815,513 B2
  • Filed: 10/31/2007
  • Issued: 08/26/2014
  • Est. Priority Date: 03/02/2001
  • Status: Expired due to Fees
First Claim
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1. A method of identifying multiple different etiologic bioagents of a disease or condition, comprising:

  • a) contacting nucleic acid from said multiple different etiologic bioagents of a disease or condition with two or more oligonucleotide primers that hybridize to sequence regions of said nucleic acid from said multiple different etiologic bioagents of a disease or condition that are conserved among different bioagents, wherein said conserved sequence regions flank variable sequence regions to produce two or more amplification products;

    b) determining the base compositions of said two or more amplification products; and

    c) identifying said multiple different etiologic bioagents of a disease or condition by comparing said determined base compositions to a database of base compositions comprising nucleic acid numbers but not nucleic acid sequences from a plurality of different bioagents.

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