Method of making cosmetic compositions containing a prebiotic
First Claim
Patent Images
1. A method of making a topical cosmetic composition, the method comprising:
- (a) providing a first culture comprising a test agent, a quantity of a human skin commensalmicroorganism, and a minimal carbon medium;
(b) determining a metabolite level or a replication level of the human skin commensal microorganism of the first culture;
(c) comparing the metabolite level or the replication level to a first control value, wherein the first control value is the metabolite level or replication level in a culture of the human skin commensal microorganism of the first culture that is grown without the test agent, and wherein the metabolite level correlates with the amount of growth;
(d) identifying the test agent as a prebiotic when the test agent increases the growth of the human skin commensal microorganism and when the metabolite level or replication level of the first culture is greater than the first control value; and
(e) combining a safe and effective amount of the prebiotic with a dermatologically acceptable carrier.
1 Assignment
0 Petitions
Accused Products
Abstract
A method for indentifying test agents that exhibit prebiotic activity on human skin commensal microorganisms and cosmetic compositions that include such agents. The method includes providing a test culture of a test agent, a human skin commensal microorganism and a minimal carbon medium. The method provides a time efficient and cost effective way to predict in vivo prebiotic activity of a test agent on skin commensal microorganisms.
61 Citations
18 Claims
-
1. A method of making a topical cosmetic composition, the method comprising:
-
(a) providing a first culture comprising a test agent, a quantity of a human skin commensal microorganism, and a minimal carbon medium; (b) determining a metabolite level or a replication level of the human skin commensal microorganism of the first culture; (c) comparing the metabolite level or the replication level to a first control value, wherein the first control value is the metabolite level or replication level in a culture of the human skin commensal microorganism of the first culture that is grown without the test agent, and wherein the metabolite level correlates with the amount of growth; (d) identifying the test agent as a prebiotic when the test agent increases the growth of the human skin commensal microorganism and when the metabolite level or replication level of the first culture is greater than the first control value; and (e) combining a safe and effective amount of the prebiotic with a dermatologically acceptable carrier. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
-
18. A method of making a topical cosmetic composition, the method comprising:
-
(a) providing a culture that includes a test agent and a quantity of a human skin commensal microorganism; (b) determining a metabolite level of the culture; (c) comparing the metabolite level of the culture to a first control value, wherein the first control value is the metabolite level in a culture of the human skin commensal microorganism culture that is grown without the test agent, and wherein the metabolite level correlates with the amount of growth; (d) performing a plate count assay using the culture when the metabolite level of the culture is greater than the first control value, wherein the greater metabolite level results from the test agent increasing the growth of the human skin commensal microorganism; (e) comparing the number of colonies present in the plate count assay to a second control value, wherein the second control value is the number of colonies in a plate count assay of a culture of the human skin commensal microorganism that is grown without the test agent; (f) identifying the test agent as a prebiotic agent if the number of colonies present in the plate count assay of step (d) is greater than the second control value; and (g) combining a safe and effective amount of the prebiotic agent with a dermatologically acceptable carrier.
-
Specification