System for fermentation of biomass for the production of ethanol

• US 8,815,552 B2
• Filed: 03/03/2010
• Issued: 08/26/2014
• Est. Priority Date: 03/03/2009
• Status: Active Grant

• Current Assignee: Poet Research Inc.
• Original Assignee: Poet Research Inc.
• Inventors: Narendranath, Neelakantam V., Carlson, David Charles
• Primary Examiner(s): Monshipouri, Maryam
• Application Number:
  US12/717,015
• Publication Number:
  US 20100227369A1
• Time in Patent Office: 1,637 Days
• Field of Search: 435/161
• US Class Current: 435/161
• CPC Class Codes: C12M 21/12 for producing fuels or solvents C12M21/04 takes precedence; liquid carbonaceous fuels C10L1/00, solid fuels C10L5/00
• CPC Class Codes: C12M 23/58 Reaction vessels connected in series or in parallel combinations of bioreactors with other apparatus, C12M43/00
• CPC Class Codes: C12M 43/02 Bioreactors or fermenters combined with devices for liquid fuel extraction; Biorefineries
• CPC Class Codes: C12M 45/02 by mechanical forces; Stirring; Trituration; Comminuting crushing, pulverizing, disintegrating in general B02C
• CPC Class Codes: C12M 45/04 Phase separators; Separation of non fermentable material; Fractionation
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A biorefinery and a system for producing a fermentation product from biomass are disclosed. The biorefinery comprises a preparation system to prepare the biomass into prepared biomass; a pre-treatment system to pre-treat the prepared biomass with a dilute acid for separation into a first component from which pentose can accessed for fermentation and a second component from which hexose can be made available for fermentation; a first treatment system to treat the first component into a treated first component by removing removed components from the first component; a first fermentation system to produce a first fermentation product from the pentose; a distillation system to recover ethanol from the first fermentation product; and a treatment system to process removed components. The biomass comprises lignocellulosic material, which comprises at least one of corn cobs, corn plant husks, corn plant leaves and corn plant stalks.

Claims

1. A method for producing a fermentation product in a fermentation system from a biomass comprising the steps of:

(i) pre-treating the biomass with an aqueous composition comprising an effective concentration of acid for reducing formation of inhibitors at a temperature of about 130°

C. to about 170°

C. for a period of time sufficient to produce a solids component and an aqueous component, which aqueous component comprises xylose and acetic acid;

(ii) supplying the aqueous component to the fermentation system;

(iii) providing yeast to the fermentation system in a concentration in the range from 5 to less than 150 grams of yeast on a dry basis per liter of the aqueous component, wherein the yeast can ferment the xylose into the fermentation product;

(iv) maintaining the aqueous component and yeast in the fermentation system at a temperature of between about 26°

C. and about 37°

C. and at a pH of between about 4.5 and about 6.0 for a time of no less than 18 hours; and

(v) recovering the fermentation product from the fermentation system;

wherein the biomass comprises lignocellulosic material.

2. The method of claim 1, wherein the lignocellulosic material consists essentially of corn cobs, corn plant husks, corn plant leaves and corn stalks.

3. The method of claim 1, wherein the aqueous component comprises xylose at about 2 to 5 percent by weight.

4. The method of claim 1, wherein the aqueous component further comprises glucose and the yeast is capable of fermenting both the xylose and glucose into ethanol.

5. The method of claim 1, wherein the yeast comprises Saccharomyces cerevisiae.

6. The method of claim 1, wherein the fermentation product comprises at least 1.5 percent ethanol by volume.

7. The method of claim 1, wherein the fermentation product comprises at least 2.0 percent ethanol by volume.

8. The method of claim 1, wherein the fermentation product comprises ethanol and wherein at least 75 percent of the xylose has been converted into ethanol by fermentation.

9. The method of claim 1, further comprising the step of treating the aqueous component to increase the concentration of xylose.

10. The method of claim 1, wherein the lignocellulosic-material comprises cellulose and lignin.

11. The method of claim 1, wherein the step of maintaining the aqueous component and yeast in the fermentation system is for a time of no less than 24 hours.

12. The method of claim 1, wherein the lignocellulosic material comprises at least one of corn cobs, corn plant husks, corn plant leaves and corn plant stalks.

13. The method of claim 1, further comprising the step of

(vii) supplying the solids component to the fermentation system;

wherein the solids component comprises hexose.

14. The method of claim 1, wherein the acid concentration is between about 0.8 and 1.1 percent by weight.

15. The method of claim 1, wherein the acid concentration is between about 0.05 and 0.5 percent by weight.

16. The method of claim 1, wherein the acetic acid is present in the aqueous component in an amount in the range from 3,000 to 6,400 parts per million.