Increased fiber hydrolysis by protease addition

• US 8,815,571 B2
• Filed: 10/08/2008
• Issued: 08/26/2014
• Est. Priority Date: 10/12/2007
• Status: Active Grant

• Current Assignee: Archer Daniels Midland Company
• Original Assignee: Archer Daniels Midland Company
• Inventors: Abbas, Charles A., Bao, Wu-Li
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): Kosar, Aaron J
• Application Number:
  US12/247,499
• Publication Number:
  US 20090098638A1
• Time in Patent Office: 2,148 Days
• US Class Current: 435/276
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/20 produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Novel fiber processing methods and the products obtained therefrom are disclosed. Methods may include thermochemical and/or enzymatic hydrolysis of fiber feedstocks including distillers'"'"' dried grains, distillers'"'"' dried grains with solubles, soyhull, miscanthus and switchgrass. Enzymatic hydrolysis includes hydrolysis with cellulase, hemicellulase, and protease.

Claims

1. A method for increasing the amount of glucose and other sugar and peptides released from a fiber containing corn or soy bean byproduct comprising:

(a) reacting a fiber containing corn or soy bean byproduct selected from the group consisting of corn gluten feed (CGF), distillers dried grains (DDG), distillers dried grains with solubles (DDGS), and soy hulls with a mixture of reactants comprising at least one protease and at least one member of the group consisting of cellulase and hemicellulase; and

(b) obtaining a reaction product from said fiber containing corn or soy byproduct and said mixture of reactants, wherein a wt/wt ratio of glucose/fiber is greater in the reaction product than the wt/wt ratio of glucose/fiber obtained from reaction of the fiber containing corn or soy processing byproduct under the same conditions as the reaction of step (a), but excluding protease, and

(c) forming an animal feed from the reaction product.

2. The method of claim 1, wherein said mixture does not include amylase.

3. The method of claim 1, wherein said protease is an acid fungal protease.

4. The method of claim 1, wherein said protease has an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:

1.

5. The method of claim 1, wherein said protease is selected from the group consisting of Aspergillus saitoi aspartic protease, Penicillium acid protease, Mucor acid protease, Monascus acid protease, Trichoderma acid protease, Phaeosphaeria acid protease, and Rhizopus acid protease.

6. The method of claim 4, wherein said protease is Aspergillus saitoi aspartic protease, said Aspergillus saitoi aspartic protease having the amino acid sequence of SEQ ID NO:

1.

7. The method of claim 1, wherein said corn or soy bean byproduct is selected from the group consisting of CGF, DDG, and DDGS.

8. The method of claim 1, wherein said fiber feedstock comprises less than 20% starch by weight.

9. The method of claim 1, wherein said fiber feedstock comprises less than 10% starch by weight.

10. The method of claim 1, wherein said fiber feedstock comprises less than 5% starch by weight.

11. The method of claim 1, wherein said fiber feedstock comprises 0% starch by weight.

12. The method of claim 1, wherein said mixture of reactants comprises cellulase, and wherein said cellulase comprises one or more of endo-β

-1,4 glucanases, exo-cellobiohydrolases, β

-glucosidase, and exoglucanases.

13. The method of claim 1, wherein said mixture of reactants comprises hemicellulase, and wherein said hemicellulase comprises one or more of endo-1,4-β

-xylanase, β

-xylosidase, β

-endomannanase, β

-mannosidase, pectin lyase, pectate lyase, α

-L-arabinofuransidase, α

-glucuronidases, α

/β

-galactosidases, and several esterases.

14. The method of claim 1, wherein said reaction product further comprises arabinose, xylose, galactose, mannose, cellulobiose, maltose, and maltotriose.

15. The method of claim 1, wherein the wt/wt ratio of glucose/fiber is greater in the reaction product than the wt/wt ratio of glucose/fiber amount of glucose obtained from reaction of the fiber containing corn or soy byproduct under the same conditions as the reaction of step (a), but excluding protease by at least 10%.

16. The method of claim 15, wherein the wt/wt ratio of glucose/fiber is greater in the reaction product than the wt/wt ratio of glucose/fiber amount of glucose obtained from reaction of the fiber containing corn or soy byproduct under the same conditions as the reaction of step (a), but excluding protease by at least 20%.

17. The method of claim 16, wherein the wt/wt ratio of glucose/fiber is greater in the reaction product than the wt/wt ratio of glucose/fiber amount of glucose obtained from reaction of the fiber containing corn or soy byproduct under the same conditions as the reaction of step (a), but excluding protease by at least 100%.

18. The method of claim 1, wherein said protease has an amino acid sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, and SEQ ID NO;

12.

19. A method for obtaining a solid hydrolyzed fiber, comprising:

(a) preparing a reaction product according to claim 1; and

(b) separating said reaction product into a solid hydrolyzed fiber fraction and a liquid fraction, wherein at least one of the hydrolyzed fiber fraction the liquid fraction are used to form the animal feed.