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Postpartum cells derived from umbilical tissue and methods of making and using the same

  • US 8,815,587 B2
  • Filed: 11/15/2010
  • Issued: 08/26/2014
  • Est. Priority Date: 06/27/2003
  • Status: Active Grant
First Claim
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1. A method for the preparation of therapeutic cells or cultures comprising:

  • a) isolating cells from human umbilical cord tissue substantially free of blood, wherein the isolated cells lack production of CD117;

    b) initially expanding the cells to a useful number in a serum-containing medium which supports cell expansion, wherein the cells produce a quantity of the cell surface marker HLA-ABC;

    c) transferring the cells to a serum-free advanced Dulbecco'"'"'s minimal essential medium containing L-glutamine and basic fibroblast growth factor (bFGF) in which the cells produce a decreased amount of the cell surface marker HLA-ABC; and

    d) passaging the cells in the serum-free medium in which the cells produce a decreased amount of the HLA-ABC,thereby preparing a therapeutic cell or culture.

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