Monitoring method, monitoring apparatus and liquid sample analyzer

• US 8,821,791 B2
• Filed: 07/24/2012
• Issued: 09/02/2014
• Est. Priority Date: 08/29/2005
• Status: Active Grant

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Shibata, Masaharu, Nakamura, Yoichi
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): TAVARES, JULIE L
• Application Number:
  US13/556,703
• Publication Number:
  US 20120288407A1
• Time in Patent Office: 770 Days
• Field of Search: 422/67, 422/73, 422/63, 422/100, 422/102
• US Class Current: 422/67
• CPC Class Codes: G01N 2035/1032 Dilution or aliquotting
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A liquid sample is aspirated using an aspirating tube provided with a flow path extending lengthwise in the interior of the tube, and an aspiration orifice for aspirating the liquid sample formed near the tip of the tube. The aspiration of the liquid sample to the aspirating tube is monitored during aspiration. Liquid sample is aspirated by the aspirating tube, and the collected material in the aspirating tube in the region at the tip side from the region containing the liquid sample to be used for measurement is delivered to a detecting part provided externally to the aspirating tube, and whether or not liquid sample is contained in the collected material is detected in the detecting part.

Claims

1. A liquid sample analyzer comprising:

a dispensing part comprising an aspiration tube having an aspiration orifice near a tip thereof for aspirating and discharging a liquid sample to be analyzed;

a measurement specimen preparing section comprising a chamber to mix a part of the liquid sample discharged from the aspiration tube and a reagent to prepare a measurement sample;

a detecting part comprising a light source and a light-receiving device;

an analyzing part configured to analyze the measurement sample prepared by the measurement specimen preparing section; and

a controller configured to;

cause the dispensing part to;

aspirate a first collected material in a first region of the aspiration tube, and a second collected material in a second region of the aspiration tube between the first region and the tip;

discharge the second collected material to the detecting part; and

discharge the first collected material into the chamber to prepare the measurement sample;

cause the detecting part to irradiate the second collected material discharged from the dispensing part with the light source and obtain optical information associated with the second collected material at the light-receiving device;

determine whether or not the first collected material is the liquid sample to be analyzed based on the optical information associated with the second collected material; and

output an alert responsive to a determination that the first collected material is not the liquid sample to be analyzed.

2. The liquid sample analyzer of claim 1, wherein the analyzing part and the detecting part are integrated.

3. The liquid sample analyzer of claim 1, wherein:

the detecting part is connected to the chamber through a first channel; and

the second collected material is discharged from the dispensing part into the chamber, then supplied from the chamber to the detecting part through the first channel.

4. The liquid sample analyzer of claim 3, wherein:

the analyzing part is connected to the chamber through a second channel; and

the first collected material is discharged from the dispensing part into the chamber, then supplied from the chamber to the analyzing part through the second channel.

5. The liquid sample analyzer of claim 1, wherein:

the dispensing part is configured to aspirate a third collected material in a third region of the aspiration tube between the first region and a base end of the aspiration tube;

the dispensing part is configured to discharge the third collected material after discharging the first collected material;

the detecting part is configured to receive the third collected material discharged by the dispensing part, irradiate the third collected material with the light source, and obtain optical information associated with the third collected material at the light-receiving device;

the controller is further configured to determine whether or not the first collected material is the liquid sample based on the optical information associated with the third collected material; and

the controller is further configured to output an alert responsive to a determination that the first collected material is not the liquid sample to be analyzed.

6. The liquid sample analyzer of claim 1, wherein the aspiration tube is configured so as to be insertable into a sealed container containing the liquid sample to be analyzed.

7. The liquid sample analyzer of claim 1, wherein the liquid sample to be analyzed comprises blood.

8. The liquid sample analyzer of claim 7, wherein the analyzing part is further configured to analyze hemoglobin in the measurement sample.

9. The liquid sample analyzer of claim 7, wherein the analyzing part is further configured to analyze blood cells in the measurement sample.

10. The liquid sample analyzer of claim 9, wherein the chamber is configured to mix the part of the sample with diluent so as to prepare the measurement sample for analyzing red blood cells in the measurement sample.

11. The liquid sample analyzer of claim 10, wherein the analyzing part is further configured to detect changes of DC resistance to count the red blood cells.

12. The liquid sample analyzer of claim 9, wherein the chamber is configured to mix the part of the sample with hemolytic reagent so as to prepare the measurement sample for analyzing white blood cells in the measurement sample.

13. The liquid sample analyzer of claim 12, wherein the analyzing part comprises an optical flow cytometer to count white blood cells.

14. A liquid sample analyzer comprising:

a dispensing part comprising an aspiration tube having an aspiration orifice near a tip thereof for aspirating and discharging a liquid sample to be analyzed;

a measurement specimen preparing section comprising a chamber to mix a part of the liquid sample discharged from the aspiration tube and a reagent to prepare a measurement sample;

a detecting part comprising a light source and a light-receiving device;

an analyzing part configured to analyze the measurement sample prepared by the measurement specimen preparing section; and

a controller configured to;

cause the dispensing part to;

aspirate a first collected material in a first region of the aspiration tube, and a second collected material in a second region of the aspiration tube between the first region and the tip;

discharge the second collected material to the detecting part; and

discharge the first collected material into the chamber to prepare the measurement sample;

cause the detecting part to irradiate the second collected material with the light source and obtain optical information associated with the second collected material at the light-receiving device;

determine whether or not the first collected material is the liquid sample to be analyzed based on the optical information associated with the second collected material;

cause the analyzing part to perform a measurement of the first collected material responsive to a determination that the first collected material is the liquid sample to be analyzed; and

output an alert responsive to a determination that the first collected material is not the liquid sample to be analyzed.