Devices and methods for multiplexed assays
First Claim
Patent Images
1. A device for assay of a fluid sample, the device comprising:
- at least first and second substantially planar members disposed in the same or parallel planes, wherein the first substantially planar member is a porous, hydrophilic, adsorbent material comprising fluid-impermeable barriers that define boundaries of plural hydrophilic regions and the second substantially planar member defines a test zone for presentation of a sample for assay;
said plural hydrophilic regions and said test zone comprising porous, hydrophilic, adsorbent material for transfer of fluid within the porous, hydrophilic, adsorbent material by capillary action;
said fluid-impermeable barriers penetrating the first planar member to define the boundaries of the plural hydrophilic regions through which fluid flows by capillary action;
said members being moveable relative to each other to permit establishment of fluid flow communication serially between at least two of said hydrophilic regions and the test zone;
a reagent disposed in said device within or in flow communication with one of said hydrophilic regions and in flow communication with said test zone when said one hydrophilic region and test zone are in fluid flow communication.
2 Assignments
0 Petitions
Accused Products
Abstract
The disclosure provides low cost, portable three-dimensional devices for performing multiplexed assays. The devices comprise at least two substantially planar layers disposed in parallel planes, wherein one of the layers is movable relative to each other parallel to the planes to permit the establishment of fluid flow communication serially between the two layers.
104 Citations
34 Claims
-
1. A device for assay of a fluid sample, the device comprising:
-
at least first and second substantially planar members disposed in the same or parallel planes, wherein the first substantially planar member is a porous, hydrophilic, adsorbent material comprising fluid-impermeable barriers that define boundaries of plural hydrophilic regions and the second substantially planar member defines a test zone for presentation of a sample for assay; said plural hydrophilic regions and said test zone comprising porous, hydrophilic, adsorbent material for transfer of fluid within the porous, hydrophilic, adsorbent material by capillary action; said fluid-impermeable barriers penetrating the first planar member to define the boundaries of the plural hydrophilic regions through which fluid flows by capillary action; said members being moveable relative to each other to permit establishment of fluid flow communication serially between at least two of said hydrophilic regions and the test zone; a reagent disposed in said device within or in flow communication with one of said hydrophilic regions and in flow communication with said test zone when said one hydrophilic region and test zone are in fluid flow communication. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
-
Specification