Modified lipids produced from oil-bearing microbial biomass and oils
First Claim
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1. A method of making a chemically modified lipid, comprising:
- (a) providing a microalgal lipid comprising no more than 500 ppm color-generating impurities, wherein the lipid is obtained from microalgae cultured under heterotrophic growth conditions in a bioreactor substantially in the absence of light; and
(b) subjecting the microalgal lipid to an epoxidation reaction to produce the modified lipid,wherein the microalgae are selected from strains Parachlorella, Prototheca, Chlorella and strains having at least 85% nucleotide sequence identity in 23S rRNA sequences to a Parachlorella, Prototheca, or Chlorella strain.
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Abstract
Provided are methods for preparing chemically modified lipids. The lipids are obtained from heterotrophically cultured microalgae and are subjected to an epoxidation reaction. The microalgae include those from the genus Parachlorella, Prototheca, Chlorella, or strains having at least 85% nucleotide sequence identity in 23S rRNA sequences to a Parachlorella, Prototheca, or Chlorella strain that are cultured in a bioreactor substantially in the absence of light.
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Citations
15 Claims
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1. A method of making a chemically modified lipid, comprising:
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(a) providing a microalgal lipid comprising no more than 500 ppm color-generating impurities, wherein the lipid is obtained from microalgae cultured under heterotrophic growth conditions in a bioreactor substantially in the absence of light; and (b) subjecting the microalgal lipid to an epoxidation reaction to produce the modified lipid, wherein the microalgae are selected from strains Parachlorella, Prototheca, Chlorella and strains having at least 85% nucleotide sequence identity in 23S rRNA sequences to a Parachlorella, Prototheca, or Chlorella strain. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification