Isolated bacterial strain of the genus;and pesticidal metabolites therefrom

• US 8,822,193 B2
• Filed: 03/15/2013
• Issued: 09/02/2014
• Est. Priority Date: 02/25/2010
• Status: Active Grant

• Current Assignee: Pro Farm Group, Inc. (Bioceres Crop Solutions Corp.)
• Original Assignee: Marrone Bio Innovations Inc. (Bioceres Crop Solutions Corp.)
• Inventors: Asolkar, Ratnakar, Koivunen, Marja, Marrone, Pamela, Huang, Huazhang, Cordova-Krevlos, Ana Lucia
• Primary Examiner(s): Ford, Allison
• Assistant Examiner(s): Paguio Frising, Michelle F
• Application Number:
  US13/843,971
• Publication Number:
  US 20140073501A1
• Time in Patent Office: 536 Days
• Field of Search: None
• US Class Current: 435/252.1
• CPC Class Codes: A01N 43/00 Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds containing cyclic anhydrides, cyclic imides A01N37/00; containing compounds of the formula containing only one heterocyclic ring, wherein m>=1 and n>=0 and is unsubstituted or alkylsubstituted pyrrolidine, piperidine, morpholine, thiomorpholine, piperazine or a polymethyleneimine with four or more CH2 groups, A01N33/00 - A01N41/12; containing cyclopropane carboxylic acids or derivatives thereof, e.g. esters having heterocyclic rings, A01N53/00
• CPC Class Codes: A01N 43/16 with oxygen as the ring hetero atom
• CPC Class Codes: A01N 43/74 five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
• CPC Class Codes: A01N 43/76 1,3-Oxazoles; Hydrogenated 1,3-oxazoles
• CPC Class Codes: A01N 43/86 six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
• CPC Class Codes: A01N 43/90 having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
• CPC Class Codes: A01N 57/20 containing acyclic or cycloaliphatic radicals
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: C07D 263/32 with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 263/34 with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 309/14 Nitrogen atoms not forming part of a nitro radical
• CPC Class Codes: C07D 407/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 413/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: C07D 413/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 493/10 Spiro-condensed systems
• CPC Class Codes: C07D 498/14 Ortho-condensed systems
• CPC Class Codes: C07D 513/04 Ortho-condensed systems
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 17/04 : containing a five-membered hetero ring, e.g. griseofulvin , vitamin C
• CPC Class Codes: C12P 17/06 : containing a six-membered hetero ring, e.g. fluorescein
• CPC Class Codes: C12P 17/14 : Nitrogen or oxygen as hetero atom and at least one other diverse hetero ring atom in the same ring
• CPC Class Codes: C12P 17/16 : containing two or more hetero rings thiamine open chain analogs C12P17/167, i.e. not condensed among themselves or through a common carbocyclic ring system
• CPC Class Codes: C12P 17/18 : containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin e.g. Rifamycin C12P17/189
• CPC Class Codes: C12R 2001/00 : Microorganisms ; Processes using microorganisms
• CPC Class Codes: C12R 2001/01 : Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Abstract

A species of Burkholderia sp with no known pathogenicity to vertebrates but with pesticidal activity (e.g., plants, insects, fungi, weeds and nematodes) is provided. Also provided are natural products derived from a culture of said species and methods of controlling pests using said natural products.

Claims

1. A method for reducing emergence and/or growth of a monocotyledonous, sedge, or dicotyledonous weed comprising applying to said weed or soil a composition comprising an isolated Burkholderia strain A396 (NRRL Accession No. B-50319) in an amount effective to reduce said emergence and/or growth of the monocotyledonous, sedge, or dicotyledonous weed, wherein the composition is applied prior to emergence of said weed.

2. The method according to claim 1, wherein said weed is selected from a grass weed, broadleaf weed, and sedge weed.

3. The method according to claim 2, wherein said grass weed is selected from Digitaria sanguinalis, Echinochloa crus-galli, and Lolium perenne.

4. The method according to claim 2, wherein said broadleaf weed is selected from Brassica kaber, Trifolium repens, Conyza canadensis, Amaranthus palmeri, and Convolvulus arvensis.

5. The method according to claim 2, wherein said sedge is Cyperus difformis.

6. The method according to claim 1, wherein the composition is a whole cell broth comprising the isolated Burkholderia strain A396.

7. The method of claim 6, wherein the composition further comprises at least one of a carrier, diluent, surfactant, or adjuvant.

8. The method according to claim 1, wherein the composition is a cell fraction, filtrate, supernatant, or extract of a whole cell broth comprising the isolated Burkholderia strain A396.

9. The method of claim 1, wherein the emergence of the weed is prevented.

10. A method of reducing growth of a monocotyledonous, sedge, or dicotyledonous weed comprising applying to said weed or soil a composition comprising an isolated Burkholderia strain A396 (NRRL Accession No. B-50319) in an amount effective to reduce said growth of the monocotyledonous, sedge, or dicotyledonous weed, wherein the composition is applied after emergence of said weed.

11. The method according to claim 10, wherein said weed is selected from a grass weed, broadleaf weed, and sedge weed.

12. The method according to claim 11, wherein said grass weed is selected from Digitaria sanguinalis and Echinochloa crus-galli.

13. The method according to claim 11, wherein said broadleaf weed is selected from Brassica kaber, Trifolium repens, Conyza canadensis, Amaranthus retroflexus, Ambrosia artemisifolia, Solanum nigrum, Medicago polymorpha, Taraxacum oficinale, Convolvulus arvensis, Chenopodium album, Rumex crispus, Malva spp., California burclovcr, Geranium dissectum, and Capsella bursapastora.

14. The method according to claim 11, wherein said sedge is Cyperus difformis.

15. The method according to claim 10, wherein the composition is a whole cell broth comprising the isolated Burkholderia strain A396.

16. The method of claim 15, wherein the composition further comprises at least one of a carrier, diluent, surfactant, or adjuvant.

17. The method according to claim 10, wherein the composition is a cell fraction, filtrate, supernatant, or extract of a whole cell broth comprising the isolated Burkholderia strain A396.

18. The method of claim 10, wherein the weed is killed.