Reactor, micro-reactor chip, micro-reactor system, and method for manufacturing the reactor

• US 8,828,321 B2
• Filed: 01/24/2006
• Issued: 09/09/2014
• Est. Priority Date: 01/26/2005
• Status: Active Grant

• Current Assignee: Seiko Instruments Incorporated (Seiko Group)
• Original Assignee: Seiko Instruments Incorporated (Seiko Group)
• Inventors: Shinohara, Yoko, Yamamoto, Minao, Shinogi, Masataka, Kato, Haruki
• Primary Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US11/338,293
• Publication Number:
  US 20060169045A1
• Time in Patent Office: 3,150 Days
• Field of Search: 422/102, 422/99, 422/68.1, 422/82.01
• US Class Current: 422/82.01
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves

Abstract

A reactor comprises a main body having a flow path substrate and a crystal substrate chemically bonded to the flow path substrate to form a flow path for running a sample to be measured and a reactor tank connected to the flow path. An adsorption film is disposed in the reactor tank for adsorbing a specific substance contained in the sample to be measured. A measuring device measures a physical quantity of the specific substance contained in the sample and adsorbed by the adsorption film.

Claims

1. A reactor comprising:

a main body having a flow path substrate made of silicone and a crystal substrate having a surface bonded to a surface of the flow path substrate, the surface of the flow path substrate having a concave portion forming with the surface of the crystal substrate a flow path for running a sample to be measured and a reactor tank connected to the flow path;

capture means disposed in the reactor tank for capturing a specific substance contained in the sample to be measured; and

a sensor configured to measure a physical quantity of the specific substance contained in the sample and captured by the capture means, the sensor including a crystal oscillator comprising at least a portion of the surface of the crystal substrate and configured to be excited by a plurality of electrodes above which the reactor tank is disposed, and including frequency measuring means connected to the plurality of electrodes for measuring a change in frequency of the crystal oscillator;

wherein the plurality of electrodes comprise a first electrode disposed on a first surface of the crystal substrate and a second electrode disposed on a second surface of the crystal substrate opposite to the first surface, the first electrode comprising a detection electrode on which the reactor tank is directly disposed.

2. A reactor according to claim 1;

wherein the sensor is configured to measure a mass of the specific substance contained in the sample.

3. A reactor according to claim 1;

wherein the crystal substrate has a mesa structure in which a region of the plurality of electrodes forms a bottom of the reactor tank.

4. A reactor according to claim 1;

wherein the frequency measuring means comprises a variable-frequency AC power supply and an ammeter, both connected in series to the plurality of electrodes of the crystal oscillator.

5. A reactor according to claim 1;

wherein the frequency measuring means comprises a frequency meter that measures an oscillation frequency of an oscillation circuit incorporating therein the crystal oscillator.

6. A reactor according to claim 1;

wherein the flow path has one or more through-holes passing through the flow path substrate for introducing into the reactor tank and discharging from the reactor tank the sample to be measured.

13. A reactor according to claim 1;

wherein the first surface of the crystal substrate is disposed in the reaction tank; and

wherein the capture means comprises an adsorption film disposed on first surface of the crystal substrate.

7. A micro-reactor chip comprising:

a flow path substrate made of silicone and having a flow path for running a sample to be measured, an introduction port for introducing the sample to be measured into a reactor tank through the flow path, and a discharge port for discharging the sample to be measured from the reactor tank through the flow path;

a crystal substrate having a surface bonded to a surface of the flow path substrate, the surface of the flow path substrate having a concave portion that forms with the surface of the crystal substrate the reactor tank;

capture means disposed in the reactor tank for capturing a specific substance contained in the sample to be measured; and

a sensor that measures a physical quantity of the specific substance contained in the sample and captured by the capture means, the sensor comprising at least a portion of the crystal substrate and including a plurality of electrodes above which the reactor tank is disposed, the plurality of electrodes comprising a first electrode disposed on a first surface of the crystal substrate and a second electrode disposed on a second surface of the crystal substrate opposite to the first surface, the first electrode comprising a detection electrode on which the reactor tank is directly disposed.

8. A micro-reactor chip according to claim 7;

wherein the flow path substrate has a valve mechanism disposed in the flow path for closing and opening the flow path.

9. A micro-reactor chip according to claim 7;

wherein the flow path substrate comprises a plurality of laminated substrate members formed with at least any of a concave portion, a groove and a through-hole.

10. A micro-reactor chip according to claim 7;

wherein the crystal substrate has a mesa structure with a predetermined region that includes the plurality of electrodes.

11. A micro-reactor chip according to claim 7;

wherein the flow path substrate has a stepped portion in a predetermined region thereof bonded to the crystal substrate for supporting the crystal substrate.

12. A micro-reactor system comprising:

a micro-reactor chip according to claim 10;

frequency measuring means connected to the plurality of electrodes of the sensor for measuring a change in frequency;

pump means connected to the introduction port or the discharge port of the flow path substrate for feeding the sample to be measured;

feed control means for controlling the opening and closing of a valve mechanism operable to open and close the flow path of the flow path substrate; and

control means for controlling the pump means, the frequency measuring means, and the feed control means.

14. A reactor comprising:

a base substrate made of silicone and having a groove, an introduction port that opens into the groove for introducing a sample to be measured, and a discharge port that opens into the groove for discharging the sample to be measured;

a crystal substrate having a surface bonded to a surface of the base substrate, the crystal substrate overlying the groove of the base substrate to form a flow path defined by the surface of the crystal substrate and the groove of the base substrate, a region of the groove of the base substrate in the chemically bonded state of the crystal substrate and the base substrate forming a reactor tank that is disposed in the flow path so that a sample to be measured that is introduced into the introduction port passes through the reactor tank and is discharged through the discharge port;

an adsorption film for adsorbing a specific substance contained in the sample to be measured; and

a measuring device that measures a physical quantity of the specific substance adsorbed by the adsorption film, the measuring device including a detection electrode above which the reactor tank is disposed, the detection electrode being disposed on the surface of the crystal substrate, and the adsorption film being disposed directly over and in contact with the detection electrode;

wherein the measuring device includes another electrode disposed on a surface of the crystal substrate opposite to the surface of the crystal substrate on which the detection electrode is disposed.

15. A reactor according to claim 14;

wherein the measuring device comprises a frequency measuring device connected to the detection electrode for measuring a change in frequency.