Method of conducting a droplet based enzymatic assay
First Claim
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1. A method of conducting a droplet-based enzymatic assay, the method comprising:
- (a) providing a droplet actuator;
(b) providing on the droplet actuator;
(i) a droplet comprising an enzyme of interest;
(ii) a droplet comprising a substrate which is potentially modified in the presence of the enzyme;
(c) executing droplet operations on the droplet actuator to combine the droplet of (b)(i) with the droplet of (b)(ii), thereby yielding an assay droplet, wherein the droplet operations are electrowetting mediated;
(d) detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator; and
wherein each of the droplet of (b)(i), the droplet of (b)(ii) and the assay droplet is at least partially surrounded by oil filler fluid.
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Abstract
A method is provided for conducting a droplet-based enzymatic assay, e.g., for diagnostic purposes. On a droplet actuator, a droplet comprising an enzyme of interest is provided along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. Droplet operations are executed to combine the enzyme and substrate droplets on the droplet actuator, thereby yielding an assay droplet on the droplet actuator. Detecting modification of the substrate by the enzyme in the assay droplet occurs on the droplet actuator. Modified substrate preparations for conducting such enzymatic assays are also provided.
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Citations
41 Claims
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1. A method of conducting a droplet-based enzymatic assay, the method comprising:
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(a) providing a droplet actuator; (b) providing on the droplet actuator; (i) a droplet comprising an enzyme of interest; (ii) a droplet comprising a substrate which is potentially modified in the presence of the enzyme; (c) executing droplet operations on the droplet actuator to combine the droplet of (b)(i) with the droplet of (b)(ii), thereby yielding an assay droplet, wherein the droplet operations are electrowetting mediated; (d) detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator; and
wherein each of the droplet of (b)(i), the droplet of (b)(ii) and the assay droplet is at least partially surrounded by oil filler fluid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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Specification