Systems and methods for detection of cells using engineered transduction particles
First Claim
1. An apparatus, comprising:
- a housing defining a channel configured to receive a sample container, the housing defining a detection volume configured to place the channel in communication with a detector, the housing including a first seal surface and a second seal surface, a first portion of the sample container and the first seal surface configured to isolate the detection volume from a volume outside of the housing when a second portion of the sample container is disposed within the detection volume; and
a shutter having a portion movably disposed within the housing between a first shutter position and a second shutter position, the shutter defining a port configured to receive at least one of a light source or a light guide, a seal surface of the shutter and the second seal surface configured to isolate the detection volume from the channel of the housing when shutter is in the first shutter position, the port in communication with the detection volume when the shutter is in the first shutter position, the channel of the housing being in communication with the detection volume when the shutter is in the second shutter position, the port being isolated from the detection volume when the shutter is in the second shutter position.
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Accused Products
Abstract
Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
101 Citations
22 Claims
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1. An apparatus, comprising:
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a housing defining a channel configured to receive a sample container, the housing defining a detection volume configured to place the channel in communication with a detector, the housing including a first seal surface and a second seal surface, a first portion of the sample container and the first seal surface configured to isolate the detection volume from a volume outside of the housing when a second portion of the sample container is disposed within the detection volume; and a shutter having a portion movably disposed within the housing between a first shutter position and a second shutter position, the shutter defining a port configured to receive at least one of a light source or a light guide, a seal surface of the shutter and the second seal surface configured to isolate the detection volume from the channel of the housing when shutter is in the first shutter position, the port in communication with the detection volume when the shutter is in the first shutter position, the channel of the housing being in communication with the detection volume when the shutter is in the second shutter position, the port being isolated from the detection volume when the shutter is in the second shutter position. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An apparatus, comprising:
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a housing defining a channel configured to receive a sample container, the housing defining a detection volume configured to place the channel in communication with a detector, the housing including a seal surface; and a shutter having a portion movably disposed within the housing between a first shutter position and a second shutter position, the shutter defining a port configured to receive at least one of a light source or a light guide, the port in communication with the detection volume when the shutter is in the first shutter position, the port being isolated from the detection volume when the shutter is in the second shutter position, the shutter including a seal surface and an actuation portion, the actuation portion configured to engage a distal end portion of the sample container to translate the shutter in a direction offset from a longitudinal axis of the channel from the first shutter position to the second shutter position when the distal end portion of the container is moved towards the detection volume, the seal surface of the shutter and the seal surface of the housing configured to isolate the detection volume from the channel of the housing when shutter is in the first shutter position, the seal surface of the shutter configured to maintain sliding contact with the seal surface of the housing when the shutter is moved from the first shutter position to the second shutter position, the channel of the housing being in communication with the detection volume when the shutter is in the second shutter position. - View Dependent Claims (14, 15, 16, 17)
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18. An apparatus, comprising:
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a housing defining a channel configured to receive a sample container, the housing including a detection window configured to place a distal end portion of the sample container in communication with a detector, the housing including a seal surface; and a shutter defining a passageway configured to receive the distal end portion of the sample container therein, the shutter movably disposed within the housing between a first shutter position and a second shutter position, the shutter defining a port configured to receive at least one of a light source or a light guide, the port in communication with the detection window when the shutter is in the first shutter position, the port being isolated from the detection window when the shutter is in the second shutter position, a seal surface of the shutter and the seal surface of the housing configured to isolate the detection window from the channel of the housing and the passageway of the shutter when shutter is in the first shutter position, the channel of the housing being aligned with the passageway of the shutter such that the distal end portion of the sample container can be received within the passageway of the shutter to place the distal end portion of the sample container in communication with the detection window when the shutter is in the second shutter position. - View Dependent Claims (19, 20, 21, 22)
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Specification