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Electrochemical detection of beta-lactoglobulin

  • US 8,834,704 B1
  • Filed: 03/15/2013
  • Issued: 09/16/2014
  • Est. Priority Date: 03/15/2013
  • Status: Active Grant
First Claim
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1. A method to measure concentration of beta-lactoglobulin (BLG), the method comprising:

  • (a) adding a known concentration of hydrogen peroxide to a sample known to, or suspected of containing BLG;

    (b) electrolyzing the sample of step (a) using a working electrode at a fixed potential sufficient to electrolyze BLG, and measuring a current signal within the sample, wherein a diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing the known concentration of hydrogen peroxide and no BLG indicates that the sample contains BLG; and

    (c) determining the concentration of BLG in the sample by comparing the diminution of the current signal in step (b) to a standard curve of current signals compiled using solutions of known BLG concentration.

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