Electrochemical detection of beta-lactoglobulin
First Claim
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1. A method to measure concentration of beta-lactoglobulin (BLG), the method comprising:
- (a) adding a known concentration of hydrogen peroxide to a sample known to, or suspected of containing BLG;
(b) electrolyzing the sample of step (a) using a working electrode at a fixed potential sufficient to electrolyze BLG, and measuring a current signal within the sample, wherein a diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing the known concentration of hydrogen peroxide and no BLG indicates that the sample contains BLG; and
(c) determining the concentration of BLG in the sample by comparing the diminution of the current signal in step (b) to a standard curve of current signals compiled using solutions of known BLG concentration.
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Abstract
A method to detect beta-lactoglobulin (BLG) is described. The method includes the steps of adding a known concentration of hydrogen peroxide to a sample known to, or suspected of containing BLG; and electrolyzing the sample using a working electrode at a fixed potential sufficient to electrolyze BLG, and measuring a current signal within the sample. A diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing a known concentration of hydrogen peroxide and no BLG indicates that the sample contains BLG.
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Citations
22 Claims
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1. A method to measure concentration of beta-lactoglobulin (BLG), the method comprising:
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(a) adding a known concentration of hydrogen peroxide to a sample known to, or suspected of containing BLG; (b) electrolyzing the sample of step (a) using a working electrode at a fixed potential sufficient to electrolyze BLG, and measuring a current signal within the sample, wherein a diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing the known concentration of hydrogen peroxide and no BLG indicates that the sample contains BLG; and (c) determining the concentration of BLG in the sample by comparing the diminution of the current signal in step (b) to a standard curve of current signals compiled using solutions of known BLG concentration. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method to detect beta-lactoglobulin (BLG), the method comprising:
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(a) adding a known concentration of hydrogen peroxide to a sample known to, or suspected of containing BLG; and (b) electrolyzing the sample of step (a) using a working electrode at a fixed potential sufficient to electrolyze BLG, and measuring a current signal within the sample, wherein a diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing the known concentration of hydrogen peroxide and no BLG indicates that the sample contains BLG. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification