Photodamage mitigation compounds and systems

• US 8,834,847 B2
• Filed: 08/11/2011
• Issued: 09/16/2014
• Est. Priority Date: 08/12/2010
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Yue, Stephen, Weber, Robert, Kong, Xiangxu, Fedorov, Andrei, Lyle, John
• Primary Examiner(s): Hartley, Michael G
• Assistant Examiner(s): LAMBERSKI, JENNIFER A
• Application Number:
  US13/208,151
• Publication Number:
  US 20120052488A1
• Time in Patent Office: 1,132 Days
• Field of Search: None
• US Class Current: 424/9.6
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12Q 1/25 involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: G01N 2333/91245 Nucleotidyltransferases (2.7.7)
• CPC Class Codes: G01N 2333/916 acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Claims

1. A reaction mixture comprising a fluorescent or fluorogenic molecule, and a photoprotective agent, which photoprotective agent comprises a reducing functional group covalently bound to an oxidizing functional group wherein the photoprotective agent comprises a compound of a formula selected from the group consisting of:

2. The reaction mixture of claim 1, further comprising an enzyme.

3. The reaction mixture of claim 2, wherein the photoprotective agent reduces an amount of photo-induced damage to the enzyme that would occur in the absence of the photoprotective agent.

4. The reaction mixture of claim 2, wherein the enzyme is a polymerase, ribosome, nuclease, or ligase enzyme.

5. The reaction mixture of claim 2, further comprising a template nucleic acid molecule.

6. The reaction mixture of claim 1, further comprising a nucleoside polyphosphate, tRNA, amino acid, or analog thereof.

7. The reaction mixture of claim 6, wherein the fluorescent or fluorogenic molecule is linked to the nucleoside polyphosphate, tRNA, amino acid, or analog thereof.

8. The reaction mixture of claim 1, wherein at least one component of the reaction mixture is confined within a zero mode waveguide.

9. The reaction mixture of claim 1, wherein the carboxylate salt is a potassium salt.

10. A compound having a formula selected from the group consisting of:

11. The compound of claim 10, wherein the carboxylate salt is a potassium salt.

12. A reaction mixture comprising a fluorescent or fluorogenic molecule, and a photoprotective agent, which photoprotective agent comprises a reducing functional group covalently bound to an oxidizing functional group, wherein the photoprotective agent comprises a compound of a formula selected from the group consisting of:

13. A compound having a formula selected from the group consisting of: