Medical implants and methods of making medical implants

• US 8,834,913 B2
• Filed: 12/28/2009
• Issued: 09/16/2014
• Est. Priority Date: 12/26/2008
• Status: Active Grant

• Current Assignee: Micell Medtech Incorporated
• Original Assignee: Battelle Memorial Institute
• Inventors: Shaw, Wendy J., Yonker, Clement R., Fulton, John L., Tarasevich, Barbara J., McClain, James B., Taylor, Doug
• Primary Examiner(s): FISHER, ABIGAIL L
• Application Number:
  US12/648,106
• Publication Number:
  US 20110159069A1
• Time in Patent Office: 1,723 Days
• Field of Search: 424/423, 424/426, 514/56
• US Class Current: 424/423
• CPC Class Codes: A61K 31/727 Heparin; Heparan
• CPC Class Codes: A61L 2300/42 Anti-thrombotic agents, anticoagulants, anti-platelet agents
• CPC Class Codes: A61L 2400/18 Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Abstract

A medical implant device having a substrate with an oxidized surface and a silane derivative coating covalently bonded to the oxidized surface. A bioactive agent is covalently bonded to the silane derivative coating. An implantable stent device including a stent core having an oxidized surface with a layer of silane derivative covalently bonded thereto. A spacer layer comprising polyethylene glycol (PEG) is covalently bonded to the layer of silane derivative and a protein is covalently bonded to the PEG. A method of making a medical implant device including providing a substrate having a surface, oxidizing the surface and reacting with derivitized silane to form a silane coating covalently bonded to the surface. A bioactive agent is then covalently bonded to the silane coating. In particular instances, an additional coating of bio-absorbable polymer and/or pharmaceutical agent is deposited over the bioactive agent.

Claims

1. A medical implant device comprising:

a device substrate having a surface;

a silane derivative coating comprising a terminal amine, wherein the silane derivative coating is covalently bonded to the surface;

a bioactive agent; and

a hetero-bifunctional polyethylene glycol spacer comprising a carboxyl group and an amine group;

wherein the spacer is between the silane derivative coating and the bioactive agent, the carboxyl group of the polyethylene glycol spacer being covalently bonded to the terminal amine of the silane derivative coating through a peptide bond and the amine group of the polyethylene glycol spacer being covalently bonded to the bioactive agent through a peptide bond;

wherein the silane derivative coating is first bonded to the surface before bonding to the polyethylene glycol spacer.

2. The device of claim 1 wherein the bioactive agent is a small molecule.

3. The device of claim 1 wherein the bioactive agent has antithrombotic activity.

4. The device of claim 3 wherein the bioactive agent is heparin.

5. The device of claim 1 wherein the surface is oxidized.

6. The device of claim 1 further comprising a bio-absorbable coating over the bioactive agent.

7. The device of claim 6 wherein the bio-absorbable coating comprises one or more polymers selected from the group consisting of poly(lactide-co-glycolide (PLGA);

poly(dl-lactide) (DLPLA);

poly(l-lactide) (LPLA);

polyglycolide (PGA);

poly(dioxanone) (PDO);

poly(glycolide-co-trimethylene carbonate) (PGA-TMC);

poly(l-lactide-co-glycolide) (PGA-LPLA);

poly(dl-lactide-co-glycolide) (PGA-DLPLA);

poly(l-lactide-co-dl-lactide) (LPLA-DLPLA); and

poly(glycolide-co-trimethylene carbonate-co-dioxanone) (PDO-PGA-TMC).

8. The device of claim 6 wherein the bio-absorbable coating comprises at least one diffusible antistenotic drug.

9. The device of claim 1 wherein the substrate comprises one or more materials selected from the group consisting of stainless steel, cobalt chromium alloy, or alternative metallic alloys.

18. The device of claim 1 or 6 wherein the bioactive agent is selected from the group consisting of biopolymers, pharmaceutical agents, synthetic polymers, amino acids and small organic molecules.

19. The device of claim 1 or 6 wherein the device is selected from the group consisting of catheters, electrodes, stents, leads, pacemakers, cardioverter or defibrillator housings, artificial joints, screws, rods, ophthalmic implants, pins, bone plates, grafts, anastomotic devices, perivascular wraps, staples, shunts, dialysis grafts, colostomy bag attachment devices, ear drainage tubes, vertebral disks, suture anchors, hemostatic barriers, clamps, plates, clips, vascular implants, tissue scaffolds, dressings, bone substitutes, intraluminal devices and vascular supports.

10. An implantable stent device comprising:

a stent core having a surface;

a layer of silane derivative comprising a terminal amine covalently bonded to the surface;

a spacer layer comprising a hetero-bifunctional polyethelene glycol (PEG) derivative comprising a carboxyl group and an amine group wherein the spacer is between the silane derivative and a protein, the carboxyl group of the spacer being covalently bonded to the terminal amine of the silane derivative through a peptide bond; and

a protein covalently bonded to the PEG amine group through a peptide bond;

wherein the silane derivative is first bonded to the surface before bonding to the polyethylene glycol spacer.

11. The device of claim 10 wherein the surface is oxidized.

12. The device of claim 10 wherein the protein comprises antithrombogenic activity.

13. The device of claim 10 further comprising at least one coating layer over the protein, wherein the at least one coating layer is bio-absorbable.

14. The device of claim 13 wherein the at least one coating layer comprises one or more antistenotic drugs.

15. The device of claim 13 wherein the at least one coating layer comprises one or more immunosuppressive drugs.

16. The device of claim 15 wherein the one or more immunosuppressive drugs is selected from the group consisting of rapamycin (sirolimus), 40-O-(2-Hydroxyethyl)rapamycin (everolimus), 40-O-Benzyl-rapamycin, 40-O-(4′

-Hydroxymethyl)benzyl-rapamycin, 40-O[4′

-(1,2-Dihydroxyethyl)]benzyl-rapamycin, 40-O-Allyl-rapamycin, 40-O-[3′

-(2,2-Dimethyl-1,3-dioxolan-4(S)-yl)-prop-2′

-en-1′

-yl]-rapamycin, (2′

;

E,4′

S)-40-O-(4′

,5′

-Dihydroxypent-2′

-en-1′

-yl)-rapamycin, 40-O-(2-Hydroxy)ethoxycar-bonylmethyl-rapamycin, 40-O-(3-Hydroxy)propyl-rapamycin, 40-O-(6-Hydroxy)hexyl-rapamycin, 40-O-[2-(2-Hydroxy)ethoxy]ethyl-rapamycin, 40-O-[(3S)-2,2-Dimethyldioxolan-3-yl]methyl-rapamycin, 40-O-[(2S)-2,3-Dihydroxyprop-1-yl]-rapamycin, 40-O-(2-Acetoxy)ethyl-rapamycin, 40-O-(2-Nicotinoyloxy)ethyl-rapamycin, 40-O-[2-(N-Morpholino)acetoxy]ethyl-rapamycin, 40-O-(2-N-Imidazolylacetoxy)ethyl-rapamycin, 40-O-[2-(N-Methyl-N′

-piperazinyl)acetoxy]ethyl-rapamycin, 39-O-Desmethyl-39,40-O,O-ethylene-rapamycin, (26R)-26-Dihydro-40-O-(2-hydroxy)ethyl-rapamycin, 28-O-Methyl-rapamycin, 40-O-(2-Nicotinamidoethyl)-rapamycin, 40-O-(2-Acetaminoethyl)-rapamycin, 40-O-(2-Tolylsulfonamidoethyl)-rapamycin, 40-O-(2-(N-Methyl-imidazo-2′

-ylcarbethoxamido)ethyl)-rapamycin, 40-O-(2-Aminoethyl)-rapamycin, 40-O-(2-Ethoxycarbonylaminoethyl)-rapamycin, 40-O-[2-(4′

,5′

-Dicarboethoxy-1′

,2′

,3′

-triazol-1′

-yl)-ethyl]-rapamycin, 42-Epi-(tetrazolyl)rapamycin (tacrolimus), and 42-[3-hydroxy-2-(hydroxymethyl)-2-methylpropanoate]rapamycin(temsirolimus).

17. The device of claim 13 wherein the at least one coating layer comprises at least one layer of bio-absorbable polymer material.

20. A method of making a medical implant device, comprising:

providing a device substrate having a surface;

oxidizing the surface to produce an oxidized surface;

reacting the oxidized surface with derivitized silane comprising a terminal amine to form a silane coating over the surface, the coating being covalently bonded to the surface;

providing a bioactive agent; and

covalently bonding heterobifunctional polyethylene glycol comprising a carboxyl group and an amine group, the carboxyl group of the spacer being covalently bonded to the terminal amine of the derivitized silane surface through a peptide bond and the amine group of the polyethylene glycol covalently bonded to the bioactive agent to form a polyethylene glycol spacer between the derivitized silane and the bioactive agent.

21. The method of claim 20 wherein the bioactive agent has antithrombotic activity.

22. The method of claim 21 wherein the bioactive agent is heparin.

23. The method of claim 20 wherein the bioactive agent is a protein and wherein the covalently bonding occurs in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride to produce a peptide bond between the terminal amine of the derivitized silane and the heterobifunctional polyethylene glycol.

24. The method of claim 20 further comprising forming a bio-absorbable coating over the bioactive agent.

25. The method of claim 24 wherein the bio-absorbable coating comprises one or more polymers selected from the group consisting of poly(lactide-co-glycolide (PLGA);

poly(dl-lactide) (DLPLA);

poly(l-lactide) (LPLA);

polyglycolide (PGA);

poly(dioxanone) (PDO);

poly(glycolide-co-trimethylene carbonate) (PGA-TMC);

poly(l-lactide-co-glycolide) (PGA-LPLA);

poly(dl-lactide-co-glycolide) (PGA-DLPLA);

poly(l-lactide-co-dl-lactide) (LPLA-DLPLA); and

poly(glycolide-co-trimethylene carbonate-co-dioxanone) (PDO-PGA-TMC).

26. The method of claim 24 wherein the bio-absorbable coating comprises at least one diffusible antistenotic drug.

27. A medical implant device comprising a stent;

at least one silane derivative comprising a terminal amine covalently bonded to a surface of the stent;

a bioactive agent; and

a hetero-bifunctional polyethylene glycol spacer comprising a carboxyl group and an amine group between the silane derivative coating and the bioactive agent, the carboxyl group of the polyethylene glycol spacer being covalently bonded to the terminal amine of the silane derivative coating through a peptide bond and the amine group of the polyethylene glycol spacer being covalently bonded to the bioactive agent through a peptide bond;

further comprising a plurality of layers that form a laminate coating on said stent;

wherein at least one of said layers comprises a bioabsorbable polymer and at least one of said layers comprises a pharmaceutical agent selected from the group consisting of rapamycin, a prodrug, a derivative, a hydrate, an ester, and a salt thereof;

wherein at least a portion of the pharmaceutical agent is in crystalline form;

wherein the silane derivative coating is first bonded to the surface of the stent before bonding to the polyethylene glycol spacer.

28. The device of claim 27 wherein said laminate coating exhibits an X-ray spectrum showing the presence of said pharmaceutical agent in crystalline form.

29. The device of claim 27 wherein said laminate coating exhibits a Raman spectrum showing the presence of said pharmaceutical agent in crystalline form.

30. The device of claim 27 wherein said laminate coating exhibits a Differential Scanning calorimetry (DSC) curve showing the presence of said pharmaceutical agent in crystalline form.

31. The device of claim 27 wherein said laminate coating exhibits Wide Angle X-ray Scattering (WAXS) spectrum showing the presence of said pharmaceutical agent in crystalline form.

32. The device of claim 27 wherein said laminate coating exhibits a wide angle radiation scattering spectrum showing the presence of said pharmaceutical agent in crystalline form.

33. The device of claim 27 wherein said laminate coating exhibits an Infra Red (IR) spectrum showing the presence of said pharmaceutical agent in crystalline form.

34. The device of claim 27 wherein the laminate coating comprises at least two polymers wherein a first polymer is PLGA copolymer with a ratio of about 40:

60 to about 60;

40 and a second polymer is a PLGA copolymer with a ratio of about 70;

30 to about 90;

10.