DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
First Claim
1. A method for determining the size distribution of cell-free DNA molecules in a sample, comprisinglabeling the cell-free DNA molecules in the sample with a fluorescent dye in a stoichiometric manner,subjecting the labeled DNA to single molecule spectroscopy, andmeasuring fluorescent burst parameters and fluorescent burst rate of the labeled DNA,wherein the fluorescent burst parameters, which comprise burst size, burst height and/or transit time, indicate the length of each of the DNA molecules;
- and the fluorescent burst rate indicates the abundance of each size of DNA molecule, andconducting single molecule DNA integrity analysis (smDIA) of the labeled DNA molecules in the sample, thereby determining the size distribution of the cell-free DNA molecules.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer.
-
Citations
28 Claims
-
1. A method for determining the size distribution of cell-free DNA molecules in a sample, comprising
labeling the cell-free DNA molecules in the sample with a fluorescent dye in a stoichiometric manner, subjecting the labeled DNA to single molecule spectroscopy, and measuring fluorescent burst parameters and fluorescent burst rate of the labeled DNA, wherein the fluorescent burst parameters, which comprise burst size, burst height and/or transit time, indicate the length of each of the DNA molecules; - and the fluorescent burst rate indicates the abundance of each size of DNA molecule, and
conducting single molecule DNA integrity analysis (smDIA) of the labeled DNA molecules in the sample, thereby determining the size distribution of the cell-free DNA molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
- and the fluorescent burst rate indicates the abundance of each size of DNA molecule, and
-
27. A method for determining the size distribution of DNA molecules in a sample, comprising
labeling the DNA molecules in the sample with a fluorescent dye in a stoichiometric manner, subjecting the labeled DNA to single molecule cylindrical illumination confocal spectroscopy (CICS), such that a uniform detection profile is produced, and measuring fluorescent burst parameters and fluorescent burst rate of the labeled DNA, wherein the fluorescent burst parameters, which comprise burst size, burst height and/or transit time, indicate the length of each of the DNA molecules; - and the fluorescent burst rate indicates the abundance of each size of DNA molecule, and
conducting single molecule DNA integrity analysis (smDIA) of the labeled DNA molecules in the sample, thereby determining the size distribution of the DNA molecules. - View Dependent Claims (28)
- and the fluorescent burst rate indicates the abundance of each size of DNA molecule, and
Specification