Supported reagents, methods, and devices

• US 8,835,157 B2
• Filed: 04/25/2008
• Issued: 09/16/2014
• Est. Priority Date: 04/25/2007
• Status: Active Grant

• Current Assignee: Diasorin Italia SPA
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Parthasarathy, Ranjani V., Danielson, Michael E., Faletti, John C., Bedingham, William
• Primary Examiner(s): Bhat, Narayan
• Application Number:
  US12/597,410
• Publication Number:
  US 20100136554A1
• Time in Patent Office: 2,335 Days
• Field of Search: 435/6.1, 435/283.1, 435/287.2, 422/68.1
• US Class Current: 435/283.1
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

Methods of providing at least one reagent for use in a device for processing sample material, delivering at least one reagent to a device for processing sample material, and adding at least one reagent to at least one of the steps in a process for detecting or assaying a nucleic acid; a support film coated with a dry reagent layer; and a device for processing sample material having a support film coated with a dry reagent layer contained within at least one chamber of the device are disclosed.

Claims

1. A device for processing sample material, the device having a plurality of chambers and channels configured for fluid delivery, the device further having channels between the chambers;

wherein at least one chamber further comprises a substrate, a first layer, a second layer, a support film on the first layer, and an adhesive layer between the support film and the first layer;

wherein the support film further comprises a dry reagent layer, or a dry reagent layer coated on a portion of the support film, and an adhesion promoting layer between the reagent layer and the support film;

wherein the support film is dimensioned to fit within and is contained within at least one chamber of the device;

wherein the dry reagent layer, which can be dissolved, dispersed, or suspended in an aqueous fluid, comprises at least one reagent which can be used in at least one of a step of sample preparation, a step of nucleic acid amplification, and a step of detection in a process for detecting or assaying a nucleic acid; and

wherein the adhesive layer is a pressure sensitive adhesive layer.

2. The device of claim 1, wherein the dry reagent layer further includes a matrix material selected from the group consisting of a water soluble polymer, a carbohydrate, and a combination thereof;

and wherein the material can be dissolved, dispersed, or suspended in water at a temperature of at least room temperature and not more than 97°

C.

3. The device of claim 1, wherein the at least one reagent is selected from the group consisting of a nucleic acid amplifying enzyme, a primer, a probe, and microspheres capable of binding a nucleic acid.

4. The device of claim 3, wherein the at least one reagent is a nucleic acid amplifying enzyme.

5. The device of claim 1, wherein the device is a microfluidic device.

6. The device of claim 1, wherein the support film coated with the dry reagent layer is adhered to a surface of the at least one chamber.

7. The device of claim 1, wherein the dry reagent layer has a water content of not more than 1% by weight of the dry reagent layer.