Analysis system
First Claim
1. A microfluidic structure comprising:
- a fluidic channel, configured to guide a flow of a fluid comprising a target analyte along a length of the fluidic channel in a first direction, the fluidic channel having a width in a second direction perpendicular to the first direction and a depth in a third direction perpendicular to the first direction and the second direction, the fluidic channel comprising a non-porous material;
a reagent pad of porous material located within the fluidic channel and having a size in the second direction substantially similar to the width of the fluidic channel wherein the reagent pad has a size in the third direction that is substantially similar to the depth of the fluidic channel; and
a sensor pad of porous material located within the fluidic channel and downstream from the reagent pad and having a size in the second direction substantially similar to the width of the fluidic channel, the reagent pad and the sensor pad being separated by a free space diffusion zone having a length in the first direction between 0.5 and 5 mm,wherein the sensor pad comprises immobilized target analyte-specific receptors that can bind the target analyte and/or a labelled product of a displacement, competition or sandwich affinity assay.
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Accused Products
Abstract
An analysis system comprises a sampling cartridge (11) comprising a housing (21, 22) having an inlet (23) for receiving a fluid sample, a sensor (26, 45), and a guide (40, 42) extending between the inlet and the sensor for guiding sample into contact with the sensor. The system also has an optical detection reader (13) for optically inspecting the sensor. The cartridge housing has an inspection window (34) and the reader (13) comprises a socket to receive the cartridge (11), and an optical system (1183, 1186) for inspecting the sensor through the window. The cartridge comprises parallel microfluidic channel (42) for flow of sample from the inlet into contact with the sensor. The sensor comprises discrete sensor pads (45) in at least one channel, with an antibody, an antigen, or molecular imprinted polymer. The channels are of microfluidic size, having a cross-sectional area in the range of about 0.3 mm2 to about 5 mm2. At least one channel comprises a reagent pad (43) upstream of the sensor (45). The inlet of the cartridge comprises an extraction chamber (23) communicating with a draining chamber having a top reservoir (61) and a bottom reservoir (63), in turn communicating with a distribution chamber (40). Together, these chambers and the interfaces between them guide sample flow in a uniform manner between the channels and also remove impurity particles and bubbles.
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Citations
56 Claims
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1. A microfluidic structure comprising:
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a fluidic channel, configured to guide a flow of a fluid comprising a target analyte along a length of the fluidic channel in a first direction, the fluidic channel having a width in a second direction perpendicular to the first direction and a depth in a third direction perpendicular to the first direction and the second direction, the fluidic channel comprising a non-porous material; a reagent pad of porous material located within the fluidic channel and having a size in the second direction substantially similar to the width of the fluidic channel wherein the reagent pad has a size in the third direction that is substantially similar to the depth of the fluidic channel; and a sensor pad of porous material located within the fluidic channel and downstream from the reagent pad and having a size in the second direction substantially similar to the width of the fluidic channel, the reagent pad and the sensor pad being separated by a free space diffusion zone having a length in the first direction between 0.5 and 5 mm, wherein the sensor pad comprises immobilized target analyte-specific receptors that can bind the target analyte and/or a labelled product of a displacement, competition or sandwich affinity assay. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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Specification