Broccoli hybrid PX 05181808 and parents thereof

• US 8,835,721 B2
• Filed: 05/16/2012
• Issued: 09/16/2014
• Est. Priority Date: 06/15/2011
• Status: Active Grant

• Current Assignee: Seminis Vegetable Seeds Incorporated (Bayer AG)
• Original Assignee: Seminis Vegetable Seeds Incorporated (Bayer AG)
• Inventors: Mero, Carl E.
• Primary Examiner(s): MCELWAIN, ELIZABETH F
• Application Number:
  US13/473,461
• Publication Number:
  US 20120324596A1
• Time in Patent Office: 853 Days
• Field of Search: None
• US Class Current: 800/306
• CPC Class Codes: A01H 5/02 Flowers
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/203 Brassica oleraceae, e.g. broccoli or kohlrabi

Abstract

The invention provides seed and plants of broccoli hybrid PX 05181808 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of broccoli hybrid PX 05181808 and the parent lines thereof, and to methods for producing a broccoli plant produced by crossing such plants with themselves or with another broccoli plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants.

Claims

1. A broccoli plant comprising at least a first set of the chromosomes of broccoli line BRM 51-1210, a sample of seed of said line having been deposited under ATCC Accession Number PTA-11868.

3. The plant of claim 1, which is inbred.

4. The plant of claim 1, which is hybrid.

7. The plant of claim 4, wherein the hybrid plant is broccoli hybrid PX 05181808, a sample of seed of said hybrid PX 05181808 having been deposited under ATCC Accession Number PTA-11824.

10. A plant part of the plant of claim 1.

11. The plant part of claim 10, further defined as a leaf, an ovule, a floret, pollen, a head, or a cell.

12. A broccoli plant having all the physiological and morphological characteristics of the broccoli plant of claim 7.

13. A tissue culture of regenerable cells of the plant of claim 1.

14. The tissue culture according to claim 13, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and stalks.

15. A broccoli plant regenerated from the tissue culture of claim 13, wherein said plant comprises all of the morphological and physiological characteristics of the broccoli plant of claim 1, when grown under the same environmental conditions.

16. A method of vegetatively propagating the plant of claim 1 comprising the steps of:

(a) collecting tissue capable of being propagated from a plant according to claim 1;

(b) cultivating said tissue to obtain proliferated shoots; and

(c) rooting said proliferated shoots to obtain rooted plantlets.

17. The method of claim 16, further comprising growing at least a first plant from said rooted plantlets.

22. The plant of claim 1, comprising a transgene.

23. The plant of claim 22, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

24. The plant of claim 1, comprising a single locus conversion.

25. The plant of claim 24, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

31. A plant part of the plant of claim 7.

32. The plant part of claim 31, further defined as a leaf, an ovule, a floret, pollen, a head, or a cell.

33. A method of producing a broccoli seed comprising crossing the plant of claim 1 with itself or a second broccoli plant and allowing seed to form.

34. A method of producing broccoli comprising:

(a) obtaining a plant according to claim 1, wherein the plant has been cultivated to maturity; and

(b) collecting broccoli from the plant.

2. A seed comprising at least a first set of the chromosomes of broccoli line BRM 51-1210, a sample of seed of said line having been deposited under ATCC Accession Number PTA-11868.

5. The seed of claim 2, which is inbred.

6. The seed of claim 2, which is hybrid.

8. The seed of claim 6, defined as a seed of broccoli hybrid PX 05181808, a sample of seed of said hybrid PX 05181808 having been deposited under ATCC Accession Number PTA-11824.

9. The seed of claim 2, defined as a seed of line BRM 51-1210.

18. A method of introducing a desired trait into a broccoli line comprising:

(a) crossing a plant of line BRM 51-1210 with a second broccoli plant that comprises a desired trait to produce F₁ progeny, a sample of seed of said line having been deposited under ATCC Accession Number PTA-11868;

(b) selecting an F₁ progeny that comprises the desired trait;

(c) backcrossing the selected F₁ progeny with a plant of line BRM 51-1210 to produce backcross progeny;

(d) selecting backcross progeny comprising the desired trait and the physiological and morphological characteristic of broccoli line BRM 51-1210; and

(e) repeating steps (c) and (d) three or more times to produce selected fourth or higher backcross progeny that comprise the desired trait and essentially all of the morphological and physiological characteristics of the broccoli plant of line BRM 51-1210, when grown under the same environmental conditions.

19. A broccoli plant produced by the method of claim 18.

20. A method of producing a broccoli plant comprising an added trait, the method comprising introducing a transgene conferring the trait into a plant of hybrid PX 05181808, or line BRM 51-1210, a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-11824, and ATCC Accession Number PTA-11868, respectively.

21. A plant produced by the method of claim 20.

26. A method for producing a seed of a plant derived from at least one of hybrid PX 05181808, or line BRM 51-1210 comprising the steps of:

(a) crossing a broccoli plant of hybrid PX 05181808, or line BRM 51-1210 with itself or a second broccoli plant;

a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-11824, and ATCC Accession Number PTA-11868, respectively; and

(b) allowing seed of a hybrid PX 05181808, or line BRM 51-1210-derived broccoli plant to form.

27. The method of claim 26, further comprising the steps of:

(c) selfing a plant grown from said hybrid PX 05181808, or BRM 51-1210-derived broccoli seed to yield additional hybrid PX 05181808, or line BRM 51-1210-derived broccoli seed;

(d) growing said additional hybrid PX 05181808, or line BRM 51-1210-derived broccoli seed of step (c) to yield additional hybrid PX 05181808, or line BRM 51-1210-derived broccoli plants; and

(e) repeating the crossing and growing steps of (c) and (d) to generate at least a first further hybrid PX 05181808, or line BRM 51-1210-derived broccoli plant.

28. The method of claim 26, wherein the second broccoli plant is of an inbred broccoli line.

29. The method of claim 26, comprising crossing line BRM 51-1210 with line BRL 51-1128, a sample of seed of said lines having been deposited under ATCC Accession Number PTA-11868, and ATCC Accession Number PTA-9674, respectively.

30. The method of claim 27, further comprising:

(f) crossing the further hybrid PX 05181808, or BRM 51-1210-derived broccoli plant with a second broccoli plant to produce seed of a hybrid progeny plant.

35. A method of producing a progeny plant comprising a single locus conversion, the method comprising obtaining a plant of line BRL 51-1128 comprising a single locus conversion and crossing the plant with a second broccoli plant, a sample of seed of said line BRL 51-1128 having been deposited under ATCC Accession No. PTA-9674.