Method for producing a biosensor

• US 8,839,961 B2
• Filed: 11/27/2006
• Issued: 09/23/2014
• Est. Priority Date: 11/25/2005
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: Nishimi, Taisei, Ezoe, Toshihide, Kuruma, Koji, Kawakami, Masayuki
• Primary Examiner(s): Menon, Krishnan S
• Application Number:
  US11/604,260
• Publication Number:
  US 20070258866A1
• Time in Patent Office: 2,857 Days
• Field of Search: None
• US Class Current: 210/500.27
• CPC Class Codes: B01D 15/361 Ion-exchange
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3208 Polymeric carriers, supports or substrates
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3221 the chemical bond being an ionic interaction
• CPC Class Codes: B01J 20/3257 the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such
• CPC Class Codes: B01J 20/3272 Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3289 Coatings involving more than one layer of same or different nature
• CPC Class Codes: B01J 47/016 Modification or after-treatment of ion-exchangers
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

Claims

1. A method for producing a biosensor, which comprises:

(i) active-esterifying a polysaccharide having a carboxyl group to obtain a polysaccharide having a carboxylic acid active ester group; and

(ii) bringing into contact, by a spin coating method, the polysaccharide having a carboxylic acid active ester group with a substrate surface coated with an organic layer having a functional group, to thereby bind the polysaccharide having a carboxylic acid active ester group to the organic layer, wherein

an aqueous solution, consisting essentially of water, and containing the polysaccharide having a carboxylic acid active ester group is coated on the substrate surface and water of the aqueous solution is evaporated to form a thin film of the polysaccharide having a carboxylic acid active ester group bound to the organic layer coated on the substrate surface,

the organic layer having a functional group is a self-assembled monolayer or one or more alkanethiols, the self-assembled monolayer having an amino group, and

the polysaccharide having a carboxylic acid active ester group is covalently bound to the organic layer on the substrate surface by a reaction between said amino group and said carboxylic acid active ester group.

2. The method according to claim 1, wherein the substrate surface coated with an organic layer having a functional group is a substrate surface coated with a mixture of alkanethiol having an amino group and alkanethiol having a hydrophilic group.

3. The method according to claim 2, wherein the hydrophilic group in the alkanethiol having a hydrophilic group is a hydroxyl group or oligoethylene glycol group.

4. The method according to claim 2, wherein the mixture of alkanethiol having an amino group and alkanethiol having a hydrophilic group comprises the alkanethiol having an amino group and the alkanethiol having a hydrophilic group at a molar ratio ranging from 1/1 to 1/1,000,000.

5. The method according to claim 2, wherein the alkanethiol having an amino group has a molecular length larger than that of the alkanethiol having a hydrophilic group.

6. The method according to claim 1, wherein the polysaccharide having a carboxyl group is a carboxymethyl dextran.

7. The method according to claim 6, wherein the polysaccharide having a carboxylic acid active ester group is brought into contact with the substrate surface by a spin coating method to form the thin film of the polysaccharide having a carboxylic acid active ester group.

8. The method according to claim 1, wherein the polysaccharide having a carboxyl group is active-esterified with EDC and NHS, or with EDC alone in Step (i).