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Culturing mammalian embryonic stem cells in human foreskin fibroblast conditioned medium

  • US 8,846,400 B2
  • Filed: 11/26/2012
  • Issued: 09/30/2014
  • Est. Priority Date: 10/07/2002
  • Status: Active Grant
First Claim
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1. A method of maintaining mammalian pluripotent stem cells in an undifferentiated state comprising culturing the mammalian pluripotent stem cells with a human foreskin fibroblast conditioned medium, wherein said foreskin fibroblast conditioned medium is of non-genetically modified human foreskin fibroblasts, and wherein said foreskin fibroblast conditioned medium comprises at least 4 ng/ml of added basic fibroblast growth factor (bFGF), and being capable of maintaining said mammalian pluripotent stem cells in an undifferentiated, proliferative state through at least 87 passages, thereby maintaining the mammalian pluripotent stem cells in the undifferentiated state.

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