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Bead incubation and washing on a droplet actuator

  • US 8,846,410 B2
  • Filed: 11/15/2013
  • Issued: 09/30/2014
  • Est. Priority Date: 04/18/2006
  • Status: Active Grant
First Claim
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1. A method of manipulating a droplet comprising magnetically responsive beads therein, the method comprising:

  • (a) providing a droplet actuator comprising;

    (i) droplet operations electrodes arranged for conducting droplet operations on a droplet operations surface;

    (ii) the droplet, arranged on the droplet operations surface and subject to droplet operations mediated by the droplet operations electrodes; and

    (ii) a magnet having a magnetic field and positioned relative to the droplet operations surface such that the droplet may be transported into the magnetic field of the magnet and transported out of the magnetic field of the magnet;

    (b) transporting the droplet into the magnetic field of the magnet by executing droplet operations mediated by the droplet operations electrodes; and

    (c) transporting the droplet out of the magnetic field of the magnet executing droplet operations mediated by the droplet operations electrodes without removing the magnet.

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