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Ultra-sensitive detection of molecules on single molecule arrays

  • US 8,846,415 B2
  • Filed: 06/19/2012
  • Issued: 09/30/2014
  • Est. Priority Date: 09/23/2008
  • Status: Active Grant
First Claim
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1. A method of quantifying a molecular species, comprising:

  • providing a plurality of particles, wherein at least some of particles are associated with at least one molecule of the molecular species;

    partitioning at least a portion of the plurality of particles across a plurality of reaction vessels so that a statistically significant fraction of the reaction vessels contain no particles associated with a molecule of the molecular species and a statistically significant fraction of reaction vessels contain at least one particle associated with at least one molecule of the molecular species;

    detecting the presence or absence of a molecule of the molecular species in at least some of the reaction vessels and determining the percentage of reaction vessels which contain a molecule of the molecular species; and

    determining a measure of the quantity of the molecular species based on the percentage of reaction vessels which contain a molecule of the molecular species.

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