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Methods and systems for image processing of microfluidic devices

  • US 8,849,037 B2
  • Filed: 11/04/2013
  • Issued: 09/30/2014
  • Est. Priority Date: 09/13/2006
  • Status: Expired due to Fees
First Claim
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1. A method of baseline correcting a ratio data curve associated with a PCR process for a microfluidic device, the method comprising:

  • providing a plurality of baseline values for the ratio data curve;

    computing a plurality of baseline removed curves by subtracting the plurality of baseline values from the ratio data curve;

    computing a plurality of fitting errors for the plurality of baseline removed curves;

    determining a minimum fitting error of the plurality of fitting errors, whereby a first baseline is characterized by having the minimum fitting error of the plurality of fitting errors; and

    computing a baseline corrected ratio data curve by removing the first baseline from the ratio data curve.

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