Concentration determination in a diffusion barrier layer
First Claim
Patent Images
1. An electrochemical sensor strip, comprising:
- a base;
a first electrode on the base, the first electrode having at least one first layer on a first conductor, the first layer including a reagent layer and a second layer between the first conductor and the first layer, the second layer having an average initial thickness of at least 1 μ
m and the second layer comprising a partially water soluble polymeric material; and
a second electrode on the base.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to improved electrochemical biosensor strips and methods for determining the concentration of an analyte in a sample. By selectively measuring a measurable species residing in a diffusion barrier layer, to the substantial exclusion of the measurable species residing exterior to the diffusion barrier layer, measurement errors introduced by sample constituents, such as red blood cells, and manufacturing variances may be reduced.
-
Citations
22 Claims
-
1. An electrochemical sensor strip, comprising:
-
a base; a first electrode on the base, the first electrode having at least one first layer on a first conductor, the first layer including a reagent layer and a second layer between the first conductor and the first layer, the second layer having an average initial thickness of at least 1 μ
m and the second layer comprising a partially water soluble polymeric material; anda second electrode on the base. - View Dependent Claims (2, 3, 4, 5, 6)
-
-
7. A method of increasing the accuracy of quantitative analyte determination comprising:
-
receiving an analyte-containing sample in an electrochemical sensor, the analyte-containing sample having a liquid component, the sensor having a first conductor, a second conductor, and a first layer on at least the first conductor, where the first layer includes a reagent layer including a binder and a mediator, and the sample provides electrical communication between the first and the second conductors; applying an electric potential between a first conductor and the second conductor in the form of a read pulse, the read pulse applied for a duration at which a measurable species within the first layer is substantially detected and the measurable species external to the first layer is not substantially detected; and determining a quantitative value of the analyte concentration in the sample in response to the read pulse, where the sample resides in a gap formed between the base and a lid of the sensor, the gap having a volume of at least 1 mL to at most 10 mL. - View Dependent Claims (8, 9, 10)
-
-
11. A method of increasing the accuracy of quantitative analyte determination comprising:
-
receiving an analyte-containing sample in an electrochemical sensor, the analyte-containing sample having a liquid component, the sensor having a first conductor, a second conductor, and a first layer on at least the first conductor, where the first layer includes a reagent layer including a binder and a mediator, and the sample provides electrical communication between the first and the second conductors; applying an electric potential between a first conductor and the second conductor in the form of a read pulse, the read pulse applied for a duration at which a measurable species within the first layer is substantially detected and the measurable species external to the first layer is not substantially detected; and determining a quantitative value of the analyte concentration in the sample in response to the read pulse, further comprising applying an initial pulse and a time delay before the read pulse, where the initial pulse is from 1 to 4 seconds. - View Dependent Claims (12)
-
-
13. An electrochemical system, for determining the concentration of an analyte in a sample, comprising:
-
a first sensor, the first sensor including; a base, a first electrode on the base, the first electrode having at least one first layer on a first conductor, the first conductor on the base, where the first layer includes at least one binder forming a diffusion barrier layer, and a second electrode; and a measurement device in electrical communication with the first electrode through the first conductor and in electrical communication with the second electrode through a second conductor; where the measurement device is capable of applying a read pulse to the first sensor and of determining a quantitative value of the analyte concentration in the sample in response to the read pulse; and a bias of the quantitative value of the analyte concentration in the sample determined with the first sensor is less than a bias of a quantitative value of the analyte concentration in the sample when determined by the measurement device with the read pulse when in electrical communication with a second sensor, where the second sensor is identical to the first sensor, but lacks the binder forming the diffusion barrier layer. - View Dependent Claims (14, 15, 16, 17, 18)
-
-
19. An electrochemical system, for determining the concentration of an analyte in a sample, comprising:
-
a first sensor, the first sensor including; a base, a first electrode on the base, the first electrode having at least one first layer on a first conductor, the first conductor on the base, where the first layer includes at least one binder forming a diffusion barrier layer, and a second electrode; and a measurement device in electrical communication with the first electrode through the first conductor and in electrical communication with the second electrode through a second conductor; where the measurement device is capable of applying a read pulse of from 1 to 10 seconds to the first sensor and of determining a quantitative value of the analyte concentration in the sample in response to the read pulse; and the accuracy of the determined quantitative value of the analyte concentration in the sample is higher when a read pulse of 1 second is applied than when a read pulse of 10 seconds is applied. - View Dependent Claims (20, 21)
-
-
22. An electrochemical system, for determining the concentration of an analyte in a sample, comprising:
-
a first plurality of sensors, each sensor of the first plurality of sensors including; a base, a first electrode on the base, the first electrode having at least one first layer on a first conductor, the first conductor on the base, where the first layer includes at least one binder forming a diffusion barrier layer, and a second electrode; and a measurement device; where, when each sensor of the first plurality of sensors is placed in electrical communication with the measurement device and an analyte-containing sample is applied to the sensor, the measurement device is capable of applying a read pulse to the sensor and determining a quantitative value of the analyte concentration in the sample in response to the read pulse, and a bias spread for the quantitative values of the analyte concentration determined from the sample by the measurement device in electrical communication with the first plurality of sensors with the read pulse for a plurality of samples having hematocrit concentrations spanning the range from 16% to 48% is less than a bias spread for the plurality of samples when determined by the measurement device in electrical communication with a second plurality of sensors with the read pulse, where the second plurality of sensors is identical to the first plurality of sensors, but lacks the binder forming the diffusion barrier layer.
-
Specification