Label free biosensors and cells

• US 8,852,876 B2
• Filed: 11/13/2012
• Issued: 10/07/2014
• Est. Priority Date: 04/05/2005
• Status: Active Grant

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Fang, Ye, Ferrie, Ann MeeJin, Fontaine, Norman Henry, Lahiri, Joydeep, Yuen, Po Ki
• Primary Examiner(s): Hanley, Susan
• Assistant Examiner(s): MARTIN, PAUL C
• Application Number:
  US13/675,396
• Publication Number:
  US 20140057310A1
• Time in Patent Office: 693 Days
• Field of Search: 435/7.21, 435/29
• US Class Current: 435/7.21
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/554 the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells

Abstract

Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in high throughput methods and can be multiplexed.

Claims

1. A method of identifying a state of a cell, the method comprising:

culturing the cell on an area of the surface of a resonant wave guide grating (RWG) biosensor in a label free optical biosensor system including a light source, and a label independent detector, to form a cell-biosensor composition; and

assaying the cell-biosensor composition, where the assaying comprises identifying the inhomogeneity of the state of the cell at the area of the surface of the RWG label-free optical biosensor where the cell is cultured.

2. The method of claim 1, wherein assaying the cell-biosensor composition differentiates between an area of the RWG biosensor surface contacting no cell and at least one of an area of the RWG biosensor surface in contact with a viable cell;

an area of the RWG biosensor surface in contact with a cell that has been transfected with a gene material;

or a combination thereof.

3. The method of claim 2, further comprising an array of RWG biosensors in a single well of a microplate, and the array of RWG biosensors are physically separated.

4. The method of claim 3, wherein the array of RWG biosensors are physically separated with a barrier, and the barrier that defines a compartment within the well is lower than the barrier defining the well in the microplate.

5. The method of claim 1, wherein identifying the inhomogeneity of the state of the cell is accomplished by:

detecting a cell adhesion-induced biosensor signal of the cell at the area of the surface of the RWG label-free optical biosensor where the cell is cultured;

or

detecting a stimulatory event-induced dynamic mass redistribution (DMR) signal in the cell at the area of the surface of the RWG label-free optical biosensor where the cell is cultured.

6. The method of claim 1, wherein the area of the surface is a portion of a single RWG biosensor, or the area is an array of RWG biosensors within a single well.

7. A method for testing a compound'"'"'s effect on a cell comprising:

culturing the cell on a resonant waveguide grating biosensor of claim 1;

washing the cell;

starving the cell;

incubating the compound with the cell; and

recording a dynamic mass redistribution (DMR) signal with the resonant waveguide grating biosensor.