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Assay cartridges and methods of using the same

  • US 8,852,922 B2
  • Filed: 08/30/2011
  • Issued: 10/07/2014
  • Est. Priority Date: 12/26/2002
  • Status: Active Grant
First Claim
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1. A method of performing a cartridge based assay, said cartridge comprising:

  • (a) a sample chamber having a sample chamber vent port, said sample chamber connected to a sample conduit comprising a first dry reagent, a first waste chamber connected through a first waste chamber conduit to said sample conduit, a first waste chamber vent port connected to said waste chamber;

    (b) a first reagent chamber containing a volume of a first liquid reagent;

    (c) a first reagent chamber vent port connected to said first reagent chamber;

    (d) a first detection chamber connected to said sample chamber by said sample conduit and to said first reagent chamber by a first reagent chamber conduit;

    (e) an air vent port connected to said sample conduit or said first reagent conduit;

    said method comprising;

    (i) moving said sample from said sample chamber into said first sample conduit by opening the sample vent port and applying a vacuum to the first waste chamber vent port;

    (ii) reconstituting said first dry reagent in said sample;

    (iii) moving a first slug of said sample having a predetermined volume into said first detection chamber by opening the air vent port and applying the vacuum to the first waste chamber vent port;

    (iv) moving said sample in said first detection chamber into said first waste chamber;

    (v) moving said first liquid reagent into said first detection chamber; and

    (vi) measuring a signal from said first detection chamber.

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