Electrochemical sensor and method for manufacturing same

• US 8,858,768 B2
• Filed: 02/04/2010
• Issued: 10/14/2014
• Est. Priority Date: 02/09/2009
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Katsuki, Koji, Fujinawa, Yoshiaki, Tsukada, Masashi, Murase, Yosuke
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US13/148,465
• Publication Number:
  US 20110308944A1
• Time in Patent Office: 1,713 Days
• Field of Search: 205/777.5, 205/778, 205/792, 20440301-40315, 600/345, 600/347
• US Class Current: 204/403.05
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 2333/902 Oxidoreductases (1.)

Abstract

The present invention provides an electrochemical sensor comprising a substrate, an electrically conductive layer made of carbon particles which is disposed on the substrate, a cell membrane mimetic structure layer containing an enzyme at least one of inside the cell membrane mimetic structure layer and on the interface of the cell membrane mimetic structure layer which is disposed on the electrically conductive layer.

Claims

1. An electrochemical sensor comprising

a substrate,

an electrically conductive layer made of carbon particles,

a cell membrane mimetic structure layer containing an enzyme at least one of inside the cell membrane mimetic structure layer and on the interface of the cell membrane mimetic structure layer,

wherein the electrically conductive layer and the cell membrane mimetic structure layer are combined and the combined layer is disosed on the substrate.

2. The electrochemical sensor according to claim 1, wherein the cell membrane mimetic structure layer is a phospholipid membrane.

3. The electrochemical sensor according to claim 2, wherein the phospholipid membrane is a 2-methacryloyloxyethyl phosphorylcholine polymer.

4. The electrochemical sensor according to claim 1, wherein the enzyme is a glucose dehydrogenase.

5. The electrochemical sensor according to claim 1, wherein the enzyme is immobilized on the cell membrane mimetic structure layer in such a state that the enzyme is self-organized with respect to the cell membrane mimetic structure layer.

6. The electrochemical sensor according to claim 1, wherein the carbon particles have a primary particle size of 100 nm or smaller and a specific surface area of 200 m²/g or larger.

7. The electrochemical sensor according to claim 1, wherein the carbon particles are Ketjen Black™

or BLACK PEARLS™

.

8. The electrochemical sensor according to claim 1, wherein the combined layer is formed by screen printing the carbon particles.

9. A method for manufacturing an electrochemical sensor comprising

preparing and mixing an ink for forming an electrically conductive layer containing carbon particles and a solution containing a phospholipid polymer for forming a cell membrane mimetic structure layer,

dispensing a drop of the mixed solution on a substrate and drying, and

immobilizing an enzyme on the cell membrane mimetic structure layer,

wherein the electrically conductive layer and the cell membrane mimetic structure layer are combined.

10. The method according to claim 9, wherein the cell membrane mimetic structure layer is a phospholipid membrane.

11. The method according to claim 10, wherein the phospholipid membrane is 2-methacryloyloxyethyl phosphorylcholine polymer.

12. The method according to claim 9, wherein the enzyme is a glucose dehydrogenase.

13. The method according to claim 9, wherein the enzyme is immobilized on the cell membrane mimetic structure layer in such a state that the enzyme is self-organized with respect to the cell membrane mimetic structure layer.

14. The method according to claim 9, wherein the carbon particles have a primary particle size of 100 nm or smaller and a specific surface area of 200m²/g or larger.

15. The method according to claim 9, wherein the carbon particles are Ketjen Black™

or BLACK PEARLS™

.

16. The method according to claim 9, wherein the combined layer is formed by screen printing the carbon particles.