Hydrogel tissue adhesive having decreased gelation time and decreased degradation time

• US 8,859,705 B2
• Filed: 11/19/2012
• Issued: 10/14/2014
• Est. Priority Date: 11/19/2012
• Status: Active Grant

• Current Assignee: Actamax Surgical Materials, LLC (DSM-Firmenich AG)
• Original Assignee: Actamax Surgical Materials, LLC (DSM-Firmenich AG)
• Inventors: Lu, Helen S. M., Blankenbicker, Cara L.
• Primary Examiner(s): Seidleck, James J
• Assistant Examiner(s): Salamon, Peter A
• Application Number:
  US13/680,801
• Publication Number:
  US 20140142063A1
• Time in Patent Office: 694 Days
• Field of Search: None
• US Class Current: 527/305
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/715 Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• CPC Class Codes: A61K 31/717 Celluloses
• CPC Class Codes: A61K 31/718 Starch or degraded starch, e.g. amylose, amylopectin
• CPC Class Codes: A61K 31/721 Dextrans
• CPC Class Codes: A61K 31/728 Hyaluronic acid
• CPC Class Codes: A61K 9/0014 Skin, i.e. galenical aspects of topical compositions non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61L 24/0031 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 24/0042 Materials resorbable by the body
• CPC Class Codes: A61L 24/08 Polysaccharides A61L24/043 takes precedence

Abstract

A hydrogel tissue adhesive having decreased gelation time and decreased degradation time is described. The hydrogel tissue adhesive is formed by reacting an oxidized polysaccharide containing aldehyde groups with a water-dispersible, multi-arm amine in the presence of a thiol additive. The thiol additive accelerates the process to form the hydrogel and accelerates the degradation of the hydrogel formed. The hydrogel may be useful as a tissue adhesive or sealant for medical applications, such as a hemostat sealant or to prevent undesired tissue-to-tissue adhesions resulting from trauma or surgery.

Claims

1. A kit for forming a hydrogel, said kit comprising:

a) a first aqueous solution or dispersion comprising;

(i) at least one oxidized polysaccharide containing aldehyde groups, having a weight-average molecular weight of about 1,000 to about 1,000,000 Daltons and having an equivalent weight per aldehyde group of about 65 to about 1,500 Daltons, said first aqueous solution or dispersion containing from about 5% to about 40% by weight of the oxidized polysaccharide; and

(ii) at least one thiol additive, wherein the thiol additive is water-dispersible, contains only one reactive thiol (SH) group, does not contain any reactive carboxylic acid, primary amine or secondary amine groups, and has a molecular weight in the range of about 60 Daltons to about 40,000 Daltons, said first aqueous solution or dispersion containing about 0.1% to about 20% by weight of the thiol additive; and

b) a second aqueous solution or dispersion comprising

(i) at least one water-dispersible, multi-arm amine wherein at least three of the arms are terminated by at least one primary amine group, said multi-arm amine having a number-average molecular weight of about 440 to about 200,000 Daltons, said second aqueous solution or dispersion containing from about 5% to about 70% by weight of the multi-arm amine;

or

(ii) at least one a water-dispersible, multi-arm amine in the form of a neat liquid wherein at least three of the arms are terminated by at least one primary amine group, said multi-arm amine having a number-average molecular weight of about 440 to about 200,000 Daltons;

wherein the hydrogel has a decreased gelation time and a decreased degradation time compared to a hydrogel prepared with said oxidized polysaccharide and said water-dispersible, multi-arm amine in the absence of the thiol additive.

2. The kit of claim 1, wherein the thiol additive is selected from the group consisting of thioglycerol, methyl thioglycolate, 2-(2-methoxyethoxy)ethane thiol, and salts of 2-mercaptoethane sulfonate.

3. The kit of claim 1, wherein the oxidized polysaccharide is selected from the group consisting of oxidized derivatives of:

dextran, carboxymethyldextran, starch, agar, cellulose, hydroxyethylcellulose, carboxymethylcellulose, pullulan, inulin, levan, and hyaluronic acid.

4. The kit of claim 1, wherein the water-dispersible, multi-arm amine is selected from the group consisting of water-dispersible multi-arm polyether amines, amino-terminated dendritic polyamidoamines, and multi-arm branched end amines.

5. The kit of claim 4, wherein the water-dispersible multi-arm polyether amines are selected from the group consisting of amino-terminated star polyethylene oxides, amino-terminated dendritic polyethylene oxides, amino-terminated comb polyethylene oxides, amino-terminated star polypropylene oxides, amino-terminated dendritic polypropylene oxides, amino-terminated comb polypropylene oxides, amino-terminated star polyethylene oxide-polypropylene oxide copolymers, amino-terminated dendritic polyethylene oxide-polypropylene oxide copolymers, amino-terminated comb polyethylene oxide-polypropylene oxide copolymers, and polyoxyalkylene triamines.

6. A hydrogel comprising the reaction product of:

a) a first aqueous solution or dispersion comprising;

(i) at least one oxidized polysaccharide containing aldehyde groups, having a weight-average molecular weight of about 1,000 to about 1,000,000 Daltons and having an equivalent weight per aldehyde group of about 65 to about 1,500 Daltons, said first aqueous solution or dispersion containing from about 5% to about 40% by weight of the oxidized polysaccharide;

and

(ii) at least one thiol additive, wherein the thiol additive is water-dispersible, contains only one reactive thiol (SH) group, does not contain any reactive carboxylic acid, primary amine or secondary amine groups, and has a molecular weight in the range of about 60 Daltons to about 40,000 Daltons, said first aqueous solution or dispersion containing about 0.1% to about 20% by weight of the thiol additive;

and

b) a second aqueous solution or dispersion comprising

(i) at least one water-dispersible, multi-arm amine wherein at least three of the arms are terminated by at least one primary amine group, said multi-arm amine having a number-average molecular weight of about 440 to about 200,000 Daltons, said second aqueous solution or dispersion containing from about 5% to about 70% by weight of the multi-arm polyether amine;

or

(ii) at least one a water-dispersible, multi-arm amine in the form of a neat liquid wherein at least three of the arms are terminated by at least one primary amine group, said multi-arm amine having a number-average molecular weight of about 440 to about 200,000 Daltons;

wherein the hydrogel has a decreased gelation time and a decreased degradation time compared to a hydrogel prepared with said oxidized polysaccharide and said water-dispersible, multi-arm amine in the absence of the thiol additive.

7. The hydrogel of claim 6, wherein the thiol additive is selected from the group consisting of thioglycerol, methyl thioglycolate, 2-(2-methoxyethoxy)ethane thiol, and salts of 2-mercaptoethane sulfonate.

8. The hydrogel of claim 6, wherein the oxidized polysaccharide is selected from the group consisting of oxidized derivatives of:

dextran, carboxymethyldextran, starch, agar, cellulose, hydroxyethylcellulose, carboxymethylcellulose, pullulan, insulin, levan, and hyaluronic acid.

9. The hydrogel of claim 6, wherein the water-dispersible, multi-arm amine is selected from the group consisting of water-dispersible multi-arm polyether amines, amino-terminated dendritic polyamidoamines, and multi-arm branched end amines.

10. The hydrogel of claim 9, wherein the water-dispersible multi-arm polyether amines are selected from the group consisting of amino-terminated star polyethylene oxides, amino-terminated dendritic polyethylene oxides, amino-terminated comb polyethylene oxides, amino-terminated star polypropylene oxides, amino-terminated dendritic polypropylene oxides, amino-terminated comb polypropylene oxides, amino-terminated star polyethylene oxide-polypropylene oxide copolymers, amino-terminated dendritic polyethylene oxide-polypropylene oxide copolymers, amino-terminated comb polyethylene oxide-polypropylene oxide copolymers, and polyoxyalkylene triamines.

11. A method for applying a hydrogel coating to an anatomical site on tissue of a living organism comprising:

applying to the site

a) a first aqueous solution or dispersion comprising;

(i) at least one oxidized polysaccharide containing aldehyde groups, having a weight-average molecular weight of about 1,000 to about 1,000,000 Daltons and having an equivalent weight per aldehyde group of about 65 to about 1,500 Daltons, said first aqueous solution or dispersion containing from about 5% to about 40% by weight of the oxidized polysaccharide;

and

(ii) at least one thiol additive, wherein the thiol additive is water-dispersible, contains only one reactive thiol (SH) group, does not contain any reactive carboxylic acid, primary amine or secondary amine groups, and has a molecular weight in the range of about 60 Daltons to about 40,000 Daltons, said first aqueous solution or dispersion containing about 0.1% to about 20% by weight of the thiol additive; and

b) a second aqueous solution or dispersion comprising

(i) at least one water-dispersible, multi-arm amine wherein at least three of the arms are terminated by at least one primary amine group, said multi-arm amine having a number-average molecular weight of about 440 to about 200,000 Daltons, said second aqueous solution or dispersion containing from about 5% to about 70% by weight of the multi-arm amine;

or

(ii) at least one water-dispersible, multi-arm amine in the form of a neat liquid wherein at least three of the arms are terminated by at least one primary amine group, said multi-arm amine having a number-average molecular weight of about 440 to about 200,000 Daltons;

wherein (a) and (b)(i) or (b) (ii) are applied to the site in any order, or (a) and (b) (i) or (b) (ii) are premixed and the resulting mixture is applied to the site before the mixture completely cures; and

wherein the hydrogel coating has a decreased gelation time and a decreased degradation time compared to a hydrogel coating prepared with said oxidized polysaccharide and said water-dispersible, multi-arm amine, in the absence of the thiol additive.

12. The method of claim 11, wherein the thiol additive is selected from the group consisting of thioglycerol, methyl thioglycolate, 2-(2-methoxyethoxy)ethane thiol, and salts of 2-mercaptoethane sulfonate.

13. The method of claim 11, wherein the oxidized polysaccharide is selected from the group consisting of oxidized derivatives of:

dextran, carboxymethyldextran, starch, agar, cellulose, hydroxyethylcellulose, carboxymethylcellulose, pullulan, inulin, levan, and hyaluronic acid.

14. The method of claim 11, wherein the water-dispersible, multi-arm amine is selected from the group consisting of water-dispersible multi-arm polyether amines, amino-terminated dendritic polyamidoamines, and multi-arm branched end amines.

15. The method of claim 14, wherein the water-dispersible multi-arm polyether amines are selected from the group consisting of amino-terminated star polyethylene oxides, amino-terminated dendritic polyethylene oxides, amino-terminated comb polyethylene oxides, amino-terminated star polypropylene oxides, amino-terminated dendritic polypropylene oxides, amino-terminated comb polypropylene oxides, amino-terminated star polyethylene oxide-polypropylene oxide copolymers, amino-terminated dendritic polyethylene oxide-polypropylene oxide copolymers, amino-terminated comb polyethylene oxide-polypropylene oxide copolymers, and polyoxyalkylene triamines.

16. A method of reducing the gelation time of a hydrogel comprising an oxidized polysaccharide and a water-dispersible, multi-arm amine, the method comprising:

a) combining the oxidized polysaccharide containing aldehyde groups, having a weight-average molecular weight of about 1,000 to about 1,000,000 Daltons and having an equivalent weight per aldehyde group of about 65 to about 1,500 Daltons, said hydrogel containing from about 5% to about 40% by weight of the oxidized polysaccharide;

with a thiol additive, wherein the thiol additive is water-dispersible, contains only one reactive thiol (SH) group, does not contain any reactive carboxylic acid, primary amine or secondary amine groups, and has a molecular weight in the range of about 60 Daltons to about 40,000 Daltons, said hydrogel containing about 0.1% to about 20% by weight of the thiol additive;

to form a combination;

b) allowing the combination to stand for a predetermined amount of time; and

c) mixing the combination of oxidized polysaccharide and thiol additive with the water-dispersible, multi-arm amine to form the hydrogel;

d) wherein the hydrogel has a decreased gelation time compared to a hydrogel prepared with said oxidized polysaccharide and said water-dispersible, multi-arm amine in the absence of the thiol additive.

17. The method of claim 16 wherein the predetermined amount of time is at least two hours.

18. A method of reducing the degradation time of a hydrogel comprising an oxidized polysaccharide and a water-dispersible, multi-arm amine, the method comprising:

a) combining the oxidized polysaccharide containing aldehyde groups, having a weight-average molecular weight of about 1,000 to about 1,000,000 Daltons and having an equivalent weight per aldehyde group of about 65 to about 1,500 Daltons, said hydrogel containing from about 5% to about 40% by weight of the oxidized polysaccharide;

with a thiol additive, wherein the thiol additive is water-dispersible, contains only one reactive thiol (SH) group, does not contain any reactive carboxylic acid, primary amine or secondary amine groups, and has a molecular weight in the range of about 60 Daltons to about 40,000 Daltons, said hydrogel containing about 0.1% to about 20% by weight of the thiol additive;

to form a combination;

b) allowing the combination to stand for a predetermined amount of time; and

c) mixing the combination of oxidized polysaccharide and thiol additive with the water-dispersible, multi-arm amine to form the hydrogel;

d) wherein the hydrogel has a decreased degradation time compared to a hydrogel prepared with said oxidized polysaccharide and said water-dispersible, multi-arm amine in the absence of the thiol additive.

19. The method of claim 18 wherein the predetermined amount of time is at least two hours.

20. An aqueous solution or dispersion comprising:

a) at least one oxidized polysaccharide containing aldehyde groups, having a weight-average molecular weight of about 1,000 to about 1,000,000 Daltons and having an equivalent weight per aldehyde group of about 65 to about 1,500 Daltons, and

b) at least one thiol additive, wherein the thiol additive is water-dispersible, contains only one reactive thiol (SH) group, does not contain any reactive carboxylic acid, primary amine or secondary amine groups, and has a molecular weight in the range of about 60 Daltons to about 40,000 Daltons.