Downy mildew resistant cucumber plants

• US 8,859,859 B2
• Filed: 04/15/2009
• Issued: 10/14/2014
• Est. Priority Date: 04/16/2008
• Status: Active Grant

• Current Assignee: Seminis Vegetable Seeds Incorporated (Bayer AG)
• Original Assignee: Seminis Vegetable Seeds Incorporated (Bayer AG)
• Inventors: Shetty, Nischit, Sipeyre, Bruno, King, Joseph, Gretenkort, Marie, van Kooten, Henk, Grit, Albert, Duran, M. Yolanda
• Primary Examiner(s): Page, Brent T
• Application Number:
  US12/424,452
• Publication Number:
  US 20090265803A1
• Time in Patent Office: 2,008 Days
• Field of Search: None
• US Class Current: 800/307
• CPC Class Codes: A01G 22/05 Fruit crops, e.g. strawberries, tomatoes or cucumbers
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: A01H 5/08 Fruits
• CPC Class Codes: A01H 6/346 Cucumis sativus[cucumber]
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention relates to cucumber lines having increased resistance to Downy Mildew. The present invention also relates to parts of cucumber plants from lines having increased Downy Mildew resistance, including seeds capable of growing cucumber plants with increased Downy Mildew resistance. Further provided are methods of producing such plants by genetic marker assisted selection.

Claims

1. A method of producing a cucumber plant having resistance to Downy Mildew comprising the steps of:

(a) crossing a cucumber plant of accession PI197088 with a second cucumber plant having at least one desired trait; and

(b) selecting at least a first progeny cucumber plant resulting from the crossing that comprises resistance to Downy Mildew and the desired trait.

2. The method of claim 1, wherein the desired trait is selected from the group consisting of fruit size, shape, color, surface appearance;

seed number, seed size, locule number;

pericarp thickness and toughness;

taste, bitterness, the presence of tubercles, shelf life, plant vigor, leaf shape, leaf length, leaf color, plant height, whether the plant is determinate or not, time to maturity, adaptation to field growth, adaptation to greenhouse growth, fruit quality acceptable to market, and resistance to one or more diseases or disease causing organisms selected from the group consisting of Verticillium wilt, root knot nematodes, Tobacco Mosaic Virus, Cucumber scab, Powdery mildew, Target spot, Cucumber Mosaic Virus and Fusarium wilt, Papaya Ringspot Virus, and Zucchini Yellow Mosaic Virus.

3. The method of claim 1, wherein selecting the first progeny comprises identifying the presence of at least a first genetic marker in the first progeny that is genetically linked to a locus contributing to Downy Mildew resistance.

4. The method of claim 3, wherein selecting the first progeny further comprises selecting the progeny based on the presence of a plurality of genetic markers from the second cucumber plant present in the progeny.

5. The method of claim 3, wherein the genetic marker is selected from the group consisting of markers CAPs_—

21826, CAPs_ENK60, CAPs_ENK59, CAPs_—

17170, CAPs_—

17179, CAPs_—

18229, CAPs_—

17563/66, and CAPs_ENK70.

6. The method of claim 5, wherein the genetic marker is selected from the group consisting of CAPs_ENK60, CAPs_—

17170, and CAPs_—

17563/66.

7. The method of claim 1, further comprising the step of:

(c) crossing the progeny plant with itself or a third plant to produce a progeny plant of a subsequent generation.

8. The method of claim 7, further comprising the steps of:

(d) crossing the progeny plant of a subsequent generation with itself or a second plant; and

(e) repeating steps (c) and (d) for an additional 3-10 generations to produce an inbred cucumber plant derived from the cucumber accession PI197088.

9. The method of claim 8, wherein said progeny plant of a subsequent generation is selected for crossing based on the presence of resistance to Downy Mildew and the desired trait.

10. The method of claim 9, wherein the progeny plant of a subsequent generation is selected at each generation for crossing based on the presence of the resistance to Downy Mildew and desired trait.

11. The method of claim 9, wherein selecting the progeny plant of a subsequent generation comprises identifying the presence of at least a first genetic marker in the first progeny that is genetically linked to a locus contributing to Downy Mildew resistance.

12. The method of claim 11, wherein selecting the progeny plant of a subsequent generation further comprises selecting the progeny based on the presence of a plurality of genetic markers from the second cucumber plant present in the progeny.

13. The method of claim 8, wherein step (e) is repeated a sufficient number of generations to obtain an inbred cucumber plant that comprises the resistance to Downy Mildew and otherwise comprises the agronomic traits of the second cucumber plant.