Tissue scaffold

• US 8,864,844 B2
• Filed: 08/09/2007
• Issued: 10/21/2014
• Est. Priority Date: 05/11/2004
• Status: Active Grant

• Current Assignee: Synthasome, Inc.
• Original Assignee: Synthasome, Inc.
• Inventors: Ratcliffe, Anthony, Kern, Andreas, Ratcliffe, Fatemeh
• Primary Examiner(s): Wax, Robert A
• Assistant Examiner(s): Park, H. Sarah
• Application Number:
  US11/890,989
• Publication Number:
  US 20070276509A1
• Time in Patent Office: 2,630 Days
• Field of Search: None
• US Class Current: 623/23.74
• CPC Class Codes: A61L 2300/406 Antibiotics
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2300/602 Type of release, e.g. controlled, sustained, slow
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: C12N 2513/00 3D culture
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0656 Adult fibroblasts

Abstract

A tissue engineered construct made totally or in part from biocompatible materials and mammalian cells and/or cell products is provided. These constructs are useful in regenerating complex tissues such as bone, ligament and tendon, which may fabricated into medical devices suitable for use in the treatment of injuries and maladies such as rotator cuff injuries, periodontal disease and hernia.

Claims

1. A three-dimensional tissue comprising a biocompatible scaffold, a continuous membrane of bioabsorbable material encasing the scaffold, and extracellular matrix deposited directly by cells onto the scaffold, wherein the three-dimensional tissue comprises no living cells, wherein the bioabsorbable material is selected from at least one member of the group consisting of glycolide, lactide, trimethylene carbonate, dioxanone, caprolactone, alkylene oxides, ortho esters, polymers and copolymers therof, collagen, hyaluronic acids, alginates, and mixtures thereof.

2. A tissue according to claim 1, further comprising at least one therapeutic agent.

3. A tissue according to claim 2, wherein the therapeutic agent is selected from the group consisting of growth factors, cytokines, lipids, guidance factors, antimicrobial agents, and antibiotics.

4. A tissue according to claim 2, wherein the therapeutic agent is VEGF or FGF4.

5. A tissue scaffold system comprising a biocompatible scaffold and a pre-formed, continuous biocompatible membrane comprising a synthetic polymer, the biocompatible membrane having an engineered surface texture, and wherein the tissue scaffold system comprises no living cells and the synthetic polymer comprises a bioabsorbable material selected from at least one member of the group consisting of glycolide, lactide, trimethylene carbonate, dioxanone, caprolactone, alkylene oxides, ortho esters, polymers and copolymers thereof, collagen, hyaluronic acids, alginates, and mixtures thereof.

6. A tissue scaffold system according to claim 5, wherein the biocompatible scaffold comprises a three dimensional network.

7. A tissue scaffold system according to claim 6, wherein the three dimensional network comprises a mesh.

8. A tissue scaffold system according to claim 6, wherein the three dimensional network comprises a member selected from the group consisting of a non-woven felt and a sponge.

9. A tissue scaffold system according to claim 5, wherein the biocompatible membrane includes a surface having ridges.

10. A tissue scaffold system according to claim 5, wherein the biocompatible membrane includes a surface having grids.

11. A tissue scaffold system according to claim 5, wherein the biocompatible membrane includes a surface having an ionic charge.

12. A tissue scaffold system according to claim 5, wherein the biocompatible membrane includes a surface having a cell attachment ligand.

13. A tissue scaffold system according to claim 5, wherein the biocompatible scaffold comprises a bioabsorbable material.

14. A tissue scaffold system according to claim 5, wherein the biocompatible scaffold comprises a non-bioabsorbable material.

15. A tissue scaffold system according to claim 14, wherein the non-bioabsorbable material is selected from at least one member of the group consisting of polypropylene, polyethylene, polyamide, polyalkylene therephalate, polyvinylidene fluoride, polytetraflouroethylene and blends and copolymers thereof.

16. A tissue scaffold system according to claim 5, wherein the biocompatible membrane is bioabsorbable.

17. A tissue scaffold system according to claim 5, wherein the scaffold is embedded in the biocompatible membrane.

18. A tissue scaffold system according to claim 5, wherein the scaffold is enveloped by the biocompatible membrane.

19. A tissue scaffold system according to claim 5, wherein the biocompatible membrane is layered with the scaffold.

20. A tissue scaffold system according to claim 5, wherein the biocompatible membrane encases the biocompatible scaffold.