Automated laboratory system

• US 8,865,474 B2
• Filed: 05/08/2006
• Issued: 10/21/2014
• Est. Priority Date: 12/18/2000
• Status: Expired due to Fees

• Current Assignee: Protedyne Corporation (Laboratory Corporation Of America Holdings)
• Original Assignee: Protedyne Corporation (Laboratory Corporation Of America Holdings)
• Inventors: Paschetto, Michael, Massaro, Peter, Boot, Jeff, Wilson, David
• Primary Examiner(s): Alexander, Lyle
• Application Number:
  US11/429,888
• Publication Number:
  US 20060201810A1
• Time in Patent Office: 3,088 Days
• Field of Search: 436/177, 422/504
• US Class Current: 436/177
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: G01N 27/44782 of a plurality of samples
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/04 Details of the conveyor system G01N35/021 - G01N35/028 take precedence
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

An automated laboratory system and method allow high-throughput and fully automated processing of materials, such as liquids including genetic materials. The invention includes a variety of aspects that may be combined into a single system. For example, processing may be performed by a plurality of robotic-equipped modular stations, where each modular station has its own unique environment in which processes are performed. Transport devices, such as conveyor belts, may move objects between modular stations, saving movement for robots in the modular stations. Gels used for gel electrophoresis may be extruded, thus decreasing the time needed to form such gels. Robotically-operated well forming tools allow wells to be formed in gels in a registered and accurate way.

Claims

1. A method for forming gels suitable for use in a gel electrophoresis process, comprising:

providing a liquid material at a first end of an enclosed extrusion cavity, the extrusion cavity having an opening at a second end opposite the first end;

forming a solidified gel in the extrusion cavity between the first and second ends by cooling the liquid material such that the liquid material solidifies to form a, solidified gel in the enclosed extrusion cavity; and

extruding the solidified gel from the opening at the second end of the enclosed extrusion cavity.

2. The method of claim 1, wherein the extrusion cavity is located within an extruder body;

and

the step of providing a liquid material includes providing the liquid material from a liquid material supply apparatus to a first end of the extruder body.

3. The method of claim 2, wherein the extrusion cavity is arranged so that the first end is lower than the second end.

4. The method of claim 2, wherein the extruder body includes a pair of spaced plates that define two sides of the extrusion cavity.

5. The method of claim 4, wherein at least one of the plates is cooled by a chilled liquid.

6. The method of claim 2, wherein the liquid material supply apparatus includes a pump that delivers the liquid material under pressure to the first end of the extrusion cavity.

7. The method of claim 6, wherein the pressure of the liquid material entering the first end of the extrusion cavity forces the gel to exit from the opening at the second end of the extrusion cavity.

8. The method of claim 2, further comprising using a cutting device to cut gels extruded from the second end of the extrusion cavity.

9. The method of claim 2, further comprising receiving a gel that exits from the opening at the second end of the extrusion cavity onto a rotatable platform.

10. The method of claim 9, further comprising evacuating air from the rotatable platform to create a suction force to secure gels to the platform.

11. The method of claim 1, wherein the step of forming a gel comprises cooling the liquid material between a pair of metal plates to form a gel.

12. The method of claim 1, wherein the step of providing a liquid material comprises providing the liquid material at a location at the first end of the extrusion cavity that is lower than the second end of the extrusion cavity.

13. The method of claim 1, further comprising:

using the extruded gel in an electrophoresis process.