Continuous monitor sensor with covalently bound enzyme
First Claim
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1. An electrochemical sensor for determining an analyte in a fluid medium, comprising:
- at least one reference electrode;
at least one working electrode comprising;
particles of an electrocatalyst in an electrode matrix, andan enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst wherein the enzyme has no covalent bonds with other components of the electrode matrix;
wherein the electrode matrix is in a porous form and the electrode matrix additionally contains a conductive electrode material.
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Abstract
The disclosure concerns a high efficiency electrochemical sensor with high signal yield for determining an analyte in a fluid medium comprising, at least one reference electrode, at least one working electrode having particles of an electrocatalyst in an electrode matrix, and an enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst. The disclosure also describes a process for producing the electrochemical sensor and a method for determining an analyte in a fluid medium using the electrochemical sensor. have a high efficiency and thus achieve a high signal yield.
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Citations
31 Claims
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1. An electrochemical sensor for determining an analyte in a fluid medium, comprising:
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at least one reference electrode; at least one working electrode comprising; particles of an electrocatalyst in an electrode matrix, and an enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst wherein the enzyme has no covalent bonds with other components of the electrode matrix; wherein the electrode matrix is in a porous form and the electrode matrix additionally contains a conductive electrode material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A method for determining an analyte in a fluid medium, comprising the steps of:
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(a) contacting the fluid medium with a reference electrode; (b) contacting the fluid medium with a working electrode comprising; particles of an electrocatalyst in an electrode matrix, and an enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst wherein the enzyme has no covalent bonds with other components of the electrode matrix; and (c) determining the presence or/and the amount of the analyte in the fluid medium by measuring a signal generated by the electrochemical sensor; wherein the electrode matrix is in a porous form and the electrode matrix additionally contains a conductive electrode material.
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Specification