Methods related to wound healing

• US 8,871,198 B2
• Filed: 03/14/2007
• Issued: 10/28/2014
• Est. Priority Date: 03/29/2006
• Status: Active Grant

• Current Assignee: Stemnion, Inc.
• Original Assignee: Stemnion, Inc.
• Inventors: Emig, Charlotte A, Trumpower, Catherine J, Marshall, Vivienne S
• Primary Examiner(s): DRISCOLL, LORA E BARNHART
• Application Number:
  US11/724,094
• Publication Number:
  US 20070231297A1
• Time in Patent Office: 2,785 Days
• US Class Current: 424/93.7
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2502/02 embryonic cells
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0629 Keratinocytes; Whole skin

Abstract

The invention is directed to methods for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived therefrom, cell products derived therefrom, each alone or in combination.

Claims

1. A method for accelerating wound healing of a treated burn wound as compared to the healing rate of a non-treated burn wound in a patient in need thereof comprising treating the burn wound by topically administering to the patient'"'"'s burn wound about 0.25-1.0×

10⁶ cultured amnion-derived epithelial cells, wherein the cultured amnion-derived epithelial cells are made by the method comprising the steps of

a) obtaining a placenta and isolating an amnion from the placenta,

b) enzymatically releasing amnion-derived epithelial cells from the amnion,

c) collecting the released amnion-derived epithelial cells, and

d) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and recombinant human EGF.

2. The method of claim 1 wherein the basal medium is IMDM.

3. The method of claim 2 wherein the IMDM is supplemented with 0.5% human serum albumin.

4. The method of claim 1 wherein the recombinant human EGF is at a concentration of 10 ng/mL of culture medium.

5. The method of claim 1 wherein the amnion-derived epithelial cells, the basal medium, and all medium supplements are free of xeno-contamination.

6. The method of claim 1 wherein the treatment increases tensile strength and breaking strength of the healing treated burn wound as compared to the tensile strength and breaking strength in a healing non-treated burn wound.

7. The method of claim 1 wherein the treatment reduces fibrosis and collagen contraction in a healing treated burn wound as compared to the fibrosis and collagen contraction in a healing non-treated burn wound.