Method for evaluation of tissue preservation solution

• US 8,871,440 B2
• Filed: 01/18/2008
• Issued: 10/28/2014
• Est. Priority Date: 03/13/2007
• Status: Active Grant

• Current Assignee: Jichi Medical University, Otsuka Pharmaceutical Factory Incorporated (Otsuka Holdings Company Limited)
• Original Assignee: Jichi Medical University, Otsuka Pharmaceutical Factory Incorporated (Otsuka Holdings Company Limited)
• Inventors: Kobayashi, Eiji
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US12/450,116
• Publication Number:
  US 20100028883A1
• Time in Patent Office: 2,475 Days
• Field of Search: 435/1.1, 435/4, 435/6.1, 536/23.1, 536/24.3
• US Class Current: 435/6.1
• CPC Class Codes: C12Q 1/66 involving luciferase
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: G01N 33/5082 Supracellular entities, e.g. tissue, organisms

Abstract

The present invention provides a method for evaluating preservative effect of a tissue preservation solution, comprising immersing a mammalian tissue introduced with a luminescence or fluorescence labeling gene in the tissue preservation solution, measuring a luminescence or fluorescence level by the labeling gene in the tissue after immersion, and evaluating the preservative effect of the tissue preservation solution based on the luminescence or fluorescence level.

Claims

1. A method for evaluating preservative effect of a tissue preservation solution for preserving mammalian tissue in a live state, comprising immersing a live mammalian tissue introduced with a luciferase gene in the tissue preservation solution, measuring a luminescence level by the luciferase gene in the tissue after immersion by contacting the tissue with a substrate solution containing luciferin and not containing ATP, and evaluating the effect of the tissue preservation solution to preserve the mammalian tissue in a live state based on the luminescence.

2. The method of claim 1, wherein the luminescence level in the tissue is measured nondisruptively.

3. The method of claim 1, wherein the tissue is isolated from a non-human mammal introduced with the luciferase gene.

4. The method of claim 3, wherein the luciferase gene is expressed ubiquitously in the mammal.

5. The method of claim 3, wherein the luciferase gene is specifically expressed in the object tissue of the mammal.

6. The method of claim 1, wherein the tissue preservation solution is a cell preservation solution.

7. The method of claim 1, wherein the tissue preservation solution is an organ preservation solution.

8. The method of claim 1, wherein the mammalian tissue is a part of an organ.

10. The method of claim 2, wherein the tissue preservation solution is a cell preservation solution.

11. The method of claim 3, wherein the tissue preservation solution is a cell preservation solution.

12. The method of claim 4, wherein the tissue preservation solution is a cell preservation solution.

13. The method of claim 5, wherein the tissue preservation solution is a cell preservation solution.

14. The method of claim 2, wherein the tissue preservation solution is an organ preservation solution.

15. The method of claim 3, wherein the tissue preservation solution is an organ preservation solution.

16. The method of claim 4, wherein the tissue preservation solution is an organ preservation solution.

17. The method of claim 5, wherein the tissue preservation solution is an organ preservation solution.

9. A method of producing a tissue preservation solution for preserving mammalian tissue in a live state having a confirmed preservative effect, comprising the following steps:

(I) mixing constituent components of a desired tissue preservation solution for preserving mammalian tissue in a live state to give the tissue preservation solution;

(II) separating a part of the tissue preservation solution obtained in (I) as a sample;

(III) immersing a live mammalian tissue introduced with a luciferase gene in the sample separated in (II);

(IV) measuring the level of luminescence by the luciferase gene in the tissue after immersion by contacting the tissue with a substrate solution containing luciferin and not containing ATP;

(V) evaluating the effect of the sample to preserve the mammalian tissue in a live state based on the luminescence level; and

(VI) obtaining, as a tissue preservation solution with confirmed effect of preserving the mammalian tissue in a live state, the tissue preservation solution from which the sample confirmed to have the desired preservative effect in (V) derives.