Modification of CXCR4 using engineered zinc finger proteins
First Claim
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1. A zinc finger protein comprising 4 zinc finger DNA-binding domains, wherein(i) each zinc finger DNA-binding domain binds to a 3 base pair target subsite;
- (ii) the zinc finger protein binds to a target site in a CXCR4 gene; and
(iii) the target site is selected from the group consisting of SEQ ID NO;
1, SEQ ID NO;
6, SEQ ID NO;
11, SEQ ID NO;
16, SEQ ID NO;
21 and SEQ ID NO;
25.
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Abstract
Disclosed herein are methods and compositions for modulating activity of CXCR4 genes, for example using zinc finger transcription factors (ZF-TFs) or zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZF-TFs or ZFNs, vectors comprising polynucleotides encoding ZF-TFs or ZFNs and cells comprising polynucleotides encoding ZF-TFs or ZFNs and/or cells comprising ZF-TF or ZFNs are also provided.
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Citations
19 Claims
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1. A zinc finger protein comprising 4 zinc finger DNA-binding domains, wherein
(i) each zinc finger DNA-binding domain binds to a 3 base pair target subsite; -
(ii) the zinc finger protein binds to a target site in a CXCR4 gene; and (iii) the target site is selected from the group consisting of SEQ ID NO;
1, SEQ ID NO;
6, SEQ ID NO;
11, SEQ ID NO;
16, SEQ ID NO;
21 and SEQ ID NO;
25. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification